Effect of polysaccharide structure on protein adsorption

被引:159
作者
McArthur, SL
McLean, KM
Kingshott, P
St John, HAW
Chatelier, RC
Griesser, HJ
机构
[1] CSIRO Mol Sci, Clayton, Vic 3169, Australia
[2] Univ New S Wales, Cooperat Res Ctr Eye Res & Technol, Sydney, NSW 2052, Australia
关键词
protein adsorption; polysaccharide; carboxymethyldextran; surface modification; plasma polymer; lysozyme; albumin; lactoferrin; immunoglobulin;
D O I
10.1016/S0927-7765(99)00086-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Using X-ray photoelectron spectroscopy for quantification, the adsorption has been studied of chicken egg lysozyme, human serum albumin (HSA), bovine colostrum lactoferrin, and gamma-globulin (IgG) from single solutions onto surface-immobilised polysaccharide coatings, which were produced by the covalent attachment of a series of carboxymethyldextrans (CMDs) onto aminated fluoropolymer surfaces. CMDs with differing degrees of carboxymethyl substitution were synthesized by the reaction of dextran with bromoacetic acid under different reactant ratios. Substantial amounts of protein adsorption onto these coatings were observed with the majority of the coating/protein combinations. On the most extensively substituted CMD (1 carboxyl group per 2 dextran units), lysozyme and lactoferrin adsorbed to approximately monolayer amounts whereas there was minimal adsorption of HSA, indicating the importance of electrostatic interfacial interactions. CMD 1:14 was similar whereas the least substituted, least dense coating, from CMD 1:30, adsorbed less lysozyme and lactoferrin but more HSA. Adsorption of the large multidomain protein IgG varied little with the coating. Grazing angle XPS data indicated that for the CMD 1:30 coating there occurred significant in-diffusion of the lower molecular weight proteins. The data suggest that elimination of adsorption of a broad spectrum of proteins is not straightforward with negatively charged polysaccharide coatings; elimination of protein accumulation onto/into such coatings may not be achievable solely with a balance of electrostatic and steric-entropic interfacial forces. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:37 / 48
页数:12
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