Integration of an immunosorbent assay system: Analysis of secretory human immunoglobulin A on polystyrene beads in a microchip

被引：365

作者：

Sato, K

Tokeshi, M

Odake, T

Kimura, H

Ooi, T

Nakao, M

Kitamori, T ^{[1
]}

机构：

[1] Kanagawa Acad Sci & Technol, Integrated Chem Project, Kawasaki, Kanagawa 2130021, Japan

[2] Juntendo Univ, Sch Med, Dept Forens Med, Bunkyo Ku, Tokyo 1138421, Japan

[3] Univ Tokyo, Dept Engn Synth, Bunkyo Ku, Tokyo 1138656, Japan

[4] Univ Tokyo, Sch Engn, Dept Appl Chem, Bunkyo Ku, Tokyo 1138656, Japan

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 06期

关键词：

D O I：

10.1021/ac991151r

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An immunosorbent assay system was integrated into a glass microchip. Polystyrene beads were introduced into a microchannel, and then human secretory immunoglobulin A (s-IgA) adsorbed on the bead surface was reacted with colloidal gold conjugated anti-s-IgA antibody and detected by a thermal lens microscope. The scale merits of liquid microspace on the molecular behavior remarkably contributed to reduced assay time. The integration cut the time necessary for the antigen-antibody reaction by 1/90, thus shortening the overall analysis time from 24 h to less than 1 h. Moreover, troublesome operations required for conventional immunosorbent assays could be replaced by simple operations.

引用

页码：1144 / 1147

页数：4

共 14 条

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