Requirement of yeast SGS1 and SRS2 genes for replication and transcription

被引：121

作者：

Lee, SK ^{[1
]}

Johnson, RE ^{[1
]}

Yu, SL ^{[1
]}

Prakash, L ^{[1
]}

Prakash, S ^{[1
]}

机构：

[1] Univ Texas, Med Branch, Sealy Ctr Mol Sci, Galveston, TX 77555 USA

来源：

SCIENCE | 1999年 / 286卷 / 5448期

关键词：

D O I：

10.1126/science.286.5448.2339

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The SGS1 gene of the yeast Saccharomyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase. Simultaneous deletion of SGS1 and SRS2 is Lethal in yeast. Here, using a conditional mutation of SGS1, it is shown that DNA replication and RNA polymerase I transcription are drastically inhibited in the srs2 Delta sgs1-ts strain at the restrictive temperature. Thus, SGS1 and SRS2 function in DNA replication and RNA polymerase I transcription. These functions may contribute to the various defects observed in Werner's and Bloom's syndromes.

引用

页码：2339 / 2342

页数：4

共 28 条

[1] Purification and characterization of the Sgs1 DNA helicase activity of Saccharomyces cerevisiae [J].

Bennett, RJ ;

Sharp, JA ;

Wang, JC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (16) :9644-9650

[2] NEED FOR DNA TOPOISOMERASE ACTIVITY AS A SWIVEL FOR DNA-REPLICATION FOR TRANSCRIPTION OF RIBOSOMAL-RNA [J].

BRILL, SJ ;

DINARDO, S ;

VOELKELMEIMAN, K ;

STERNGLANZ, R .

NATURE, 1987, 326 (6111) :414-416

[3] THE TATA-BINDING PROTEIN IS REQUIRED FOR TRANSCRIPTION BY ALL 3 NUCLEAR-RNA POLYMERASES IN YEAST-CELLS [J].

CORMACK, BP ;

STRUHL, K .

CELL, 1992, 69 (04) :685-696

[4] Human genetics - Mutation-causing mutations [J].

Ellis, NA .

NATURE, 1996, 381 (6578) :110-111

[5] THE BLOOMS-SYNDROME GENE-PRODUCT IS HOMOLOGOUS TO RECQ HELICASES [J].

ELLIS, NA ;

GRODEN, J ;

YE, TZ ;

STRAUGHEN, J ;

LENNON, DJ ;

CIOCCI, S ;

PROYTCHEVA, M ;

GERMAN, J .

CELL, 1995, 83 (04) :655-666

[6] THE YEAST TYPE-I TOPOISOMERASE TOP3 INTERACTS WITH SGS1, A DNA HELICASE HOMOLOG - A POTENTIAL EUKARYOTIC REVERSE GYRASE [J].

GANGLOFF, S ;

MCDONALD, JP ;

BENDIXEN, C ;

ARTHUR, L ;

ROTHSTEIN, R .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (12) :8391-8398

[7] BLOOMS SYNDROME [J].

GERMAN, J .

DERMATOLOGIC CLINICS, 1995, 13 (01) :7-18

[8] The Werner syndrome protein is a DNA helicase [J].

Gray, MD ;

Shen, JC ;

KamathLoeb, AS ;

Blank, A ;

Sopher, BL ;

Martin, GM ;

Oshima, J ;

Loeb, LA .

NATURE GENETICS, 1997, 17 (01) :100-103

[9] DNA-REPAIR GENE RAD3 OF SACCHAROMYCES-CEREVISIAE IS ESSENTIAL FOR TRANSCRIPTION BY RNA POLYMERASE-II [J].

GUZDER, SN ;

QIU, HF ;

SOMMERS, CH ;

SUNG, P ;

PRAKASH, L ;

PRAKASH, S .

NATURE, 1994, 367 (6458) :91-94

[10] ISOLATION AND CHARACTERIZATION OF TEMPERATURE-SENSITIVE RNA POLYMERASE-II MUTANTS OF SACCHAROMYCES-CEREVISIAE [J].

HIMMELFARB, HJ ;

SIMPSON, EM ;

FRIESEN, JD .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (06) :2155-2164

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