Evaluation of adenosine triphosphate (ATP) bioluminescence for estimating bacteria on surfaces of beef carcasses

被引：6

作者：

Bautista, DA

Kozub, G

Jericho, KWF

Griffiths, MW

机构：

[1] UNIV GUELPH,DEPT FOOD SCI,GUELPH,ON N1G 2W1,CANADA

[2] AGR & AGRI FOOD CANADA,RES BRANCH,RES STN,STAT UNIT,LETHBRIDGE,AB T1J 4B12,CANADA

[3] AGR & AGRI FOOD CANADA,FOOD PROD & INSPECT BRANCH,INST ANIM DIS RES,LETHBRIDGE,AB T1J 3Z4,CANADA

来源：

JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY | 1997年 / 5卷 / 01期

关键词：

D O I：

10.1111/j.1745-4581.1997.tb00147.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A rapid (<15 min), inexpensive and simple method has been developed to estimate the concentration of bacteria on surfaces of beef carcasses using adenosine triphosphate (ATP) bioluminescence. Surfaces (5 x 5 cm(2)) of beef carcasses (n = 159) were collected by excision. An ATP assay and aerobic plate count were performed on each sample. A significant (p < 0.001) positive linear relationship (r = 0.83) between plate count and ATP assay was obtained for 159 beef carcass samples. When thresholds levels were set at 1 x 10(4), 1 x 10(5) and 1 x 10(6) CFU/cm(2), there was moderate to good agreement between the ATP bioluminescence assay and the aerobic plate count as determined by the kappa-statistic. The application of this ATP bioluminescence test to HACCP systems for beef slaughter processes is discussed.

引用

页码：37 / 45

页数：9

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