Competition between SsrA tagging and translational termination at weak stop codons in Escherichia coli

被引:53
作者
Collier, J [1 ]
Binet, E [1 ]
Bouloc, P [1 ]
机构
[1] Univ Paris 11, Inst Genet & Microbiol, Lab Reseaux Regulat, CNRS,UMR 8621, F-91405 Orsay, France
关键词
D O I
10.1046/j.1365-2958.2002.03045.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
SsrA is a tmRNA involved in tagging polypeptides on stalled ribosomes. The resulting fusion proteins are then degraded. We purified endogenous SsrA-tagged proteins by means of a genetically engineered SsrA and identified some of them. Analysis of the proteins suggested that they are tagged at their C-terminal extremities. One of them, ribokinase, is expressed from a messenger with a poorly efficient stop codon, leading to translational recoding events. A change in the ribokinase coding sequence from a weak to a strong translational stop sequence (UGAc to UAAu) annihilated SsrA tagging. Translational termination by UGA recruits the translational release factor (RF) 2. We observed that SsrA tagging of ribokinase was inversely correlated with RF2 activity, revealing a dynamic competition between translational termination and SsrA tagging.
引用
收藏
页码:745 / 754
页数:10
相关论文
共 48 条
  • [1] SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon
    Abo, T
    Inada, T
    Ogawa, A
    Aiba, H
    [J]. EMBO JOURNAL, 2000, 19 (14) : 3762 - 3769
  • [2] COMPETITION BETWEEN FRAMESHIFTING, TERMINATION AND SUPPRESSION AT THE FRAMESHIFT SITE IN THE ESCHERICHIA-COLI RELEASE FACTOR-II MESSENGER-RNA
    ADAMSKI, FM
    DONLY, BC
    TATE, WP
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (22) : 5074 - 5078
  • [3] THE CONCENTRATION OF POLYPEPTIDE-CHAIN RELEASE FACTOR-1 AND FACTOR-2 AT DIFFERENT GROWTH-RATES OF ESCHERICHIA-COLI
    ADAMSKI, FM
    MCCAUGHAN, KK
    JORGENSEN, F
    KURLAND, CG
    TATE, WP
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1994, 238 (03) : 302 - 308
  • [4] RECODE:: a database of frameshifting, bypassing and codon redefinition utilized for gene expression
    Baranov, PV
    Gurvich, OL
    Fayet, O
    Prère, MF
    Miller, WA
    Gesteland, RF
    Atkins, JF
    Giddings, MC
    [J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (01) : 264 - 267
  • [5] Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease
    Bohn, C
    Binet, E
    Bouloc, P
    [J]. MOLECULAR GENETICS AND GENOMICS, 2002, 266 (05) : 827 - 831
  • [6] Bollag DM, 1991, PROTEIN METHODS
  • [7] BACTERIAL PEPTIDE-CHAIN RELEASE FACTORS - CONSERVED PRIMARY STRUCTURE AND POSSIBLE FRAMESHIFT REGULATION OF RELEASE FACTOR-II
    CRAIGEN, WJ
    COOK, RG
    TATE, WP
    CASKEY, CT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (11) : 3616 - 3620
  • [8] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
    Datsenko, KA
    Wanner, BL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) : 6640 - 6645
  • [9] The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins
    Einhauer, A
    Jungbauer, A
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 49 (1-3): : 455 - 465
  • [10] Eubacterial tmRNAs: everywhere except the alpha-Proteobacteria?
    Felden, B
    Gesteland, RF
    Atkins, JF
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1999, 1446 (1-2): : 145 - 148