Serum markers of oxidative stress and severity of diabetic retinopathy

OBJECTIVE - To compare serum markers of oxidative stress with diabetic retinopathy seventy. RESEARCH DESIGN AND METHODS - This cross-sectional study compared patients with types 1 and 2 diabetes with control subjects in western New York and Pennsylvania. Retinopathy severity was graded from funduscopic fields based on the Early Treatment of Diabetic Retinopathy Study Serum samples were analyzed for thiobarbituric acid-reacting substances (TBARS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, creatinine, HbA(1), and triglycerides. Appropriate analysis of covariance models were performed. RESULTS- TBARS (P = 0.019), triglyceride (P = 0.004), and glucose and HbA(1) (both P < 0.001) levels were elevated in diabetic patients compared with those in control subjects. SOD (P = 0.003) and GSH-Px (P = 0.046) levels were lower in diabetic patients than in control subjects, No correlation existed between SOD levels and either glucose or HbA(1), levels. No significant associations existed between levels of TEARS, SOD, or GSH-Px an severity of diabetic retinopathy There was a significant association between poorer visual acuity and worse retinopathy (P = 0.009), which was only partly explained by macular edema. CONCLUSIONS - increased levels of TEARS and decreased levels of SOD and GSH-Px were found in diabetic patients compared with those in control subjects, but no significant associations were found between the levels of these substances and severity of retinopathy. When duration and type of diabetes and serum HbA(1) levels were taken into account, only visual acuity remained associated with more severe retinopathy.