Real-time PCR for quantification of eleven individual Fusarium species in cereals

被引：227

作者：

Nicolaisen, Mogens ^{[1
]}

Supronien, Skaidre

Nielsen, Linda Kaergaard ^{[1
]}

Lazzaro, Irene ^{[1
]}

Spliid, Niels Henrik ^{[1
]}

Justesen, Annemarie Feier ^{[1
]}

机构：

[1] Aarhus Univ, Fac Agr Sci, Dept Integrated Pest Management, DK-4200 Slagelse, Denmark

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 76卷 / 03期

关键词：

Fusarium; Mycotoxin; Real-time PCR; Maize; Wheat; HEAD BLIGHT; MYCOTOXINS; WHEAT; DNA;

D O I：

10.1016/j.mimet.2008.10.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Contamination of cereals with Fusarium species is one of the major sources of mycotoxins in food and feed. Quantification of biomass of Fusarium species is essential to understand the interactions of individual species in disease development. In this study quantitative real-time PCR assays based on the elongation factor 1 alpha (EF1 alpha) gene for the 11 Fusarium species F. graminearum, F. culmorum, F. poae, F. langsethiae, F. sporotrichioides, F. equiseti, F. tnicinctum, F. avenaceum, F. verticillioides, F. subglutinans and F. proliferatum were developed and tested on 24 wheat and 24 maize field samples. The assays were found to be specific and sensitive. Generally, the results from the quantitative real-time PCR assays corresponded well with mycotoxin data of the field samples. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：234 / 240

页数：7

共 16 条

[1]

Bottalico A., 1998, Journal of Plant Pathology, V80, P85

[2] Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe [J].

Bottalico, A ;

Perrone, G .

EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2002, 108 (07) :611-624

[3] Fusarium mycotoxins:: a review of global implications for animal health, welfare and productivity [J].

D'Mello, JPF ;

Placinta, CM ;

Macdonald, AMC .

ANIMAL FEED SCIENCE AND TECHNOLOGY, 1999, 80 (3-4) :183-205

[4] Method evaluation of Fusarium DNA extraction from mycelia and wheat for down-stream real-time PCR quantification and correlation to mycotoxin levels [J].

Fredlund, Elisabeth ;

Gidlund, Ann ;

Olsen, Monica ;

Borjesson, Thomas ;

Spliid, Niels Henrik ;

Simonsson, Magnus .

JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 73 (01) :33-40

[5] The prevalence and distribution of Fusarium species in Norwegian cereals:: a survey [J].

Kosiak, B ;

Torp, M ;

Skjerve, E ;

Thrane, U .

ACTA AGRICULTURAE SCANDINAVICA SECTION B-SOIL AND PLANT SCIENCE, 2003, 53 (04) :168-176

[6] Phylogeny and toxigenic potential is correlated in Fusarium species as revealed by partial translation elongation factor 1 alpha gene sequences [J].

Kristensen, R ;

Torp, M ;

Kosiak, B ;

Holst-Jensen, A .

MYCOLOGICAL RESEARCH, 2005, 109 :173-186

[7] The occurrence of HT-2 toxin and other trichothecenes in Norwegian cereals [J].

Langseth W. ;

Rundberget T. .

Mycopathologia, 1999, 147 (3) :157-165

[8] Toxigenic Fusarium species and mycotoxins associated with maize ear rot in Europe [J].

Logrieco, A ;

Mulè, G ;

Moretti, A ;

Bottalico, A .

EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2002, 108 (07) :597-609

[9] Epidemiology of Fusarium diseases and their mycotoxins in maize ears [J].

Munkvold, GP .

EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2003, 109 (07) :705-713

[10] PCR-based diagnosis and quantification of mycotoxin producing fungi [J].

Niessen, Ludwig .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 119 (1-2) :38-46

← 1 2 →