Composition of sulfated glycosaminoglycans and immunodistribution of chondroitin sulfate in deeply infiltrating endometriosis affecting the rectosigmoid

被引：7

作者：

Berardo, Plinio T. ^{[1
]}

Abrao, Mauricio S. ^{[2
]}

Souza, Maisa L. S. ^{[3
,4
]}

Machado, Daniel E. ^{[1
]}

Silva, Luiz-Claudio F. ^{[3
,4
]}

Nasciutti, Luiz E. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Dept Histol & Embriol, Inst Ciencias Biomed, BR-21941590 Rio De Janeiro, Brazil

[2] Univ Sao Paulo, Fac Med, Dept Obstet & Ginecol, Sao Paulo, Brazil

[3] Hosp Univ Clementino Fraga Filho, Lab Tecido Conjuntivo, Rio De Janeiro, Brazil

[4] Univ Fed Rio de Janeiro, Inst Bioquim Med, BR-21941590 Rio De Janeiro, Brazil

来源：

MICRON | 2009年 / 40卷 / 5-6期

关键词：

Chondroitin sulfate; Glycosaminoglycan; Human endometrium; Deeply infiltrating endometriosis; MENSTRUAL-CYCLE; IMPLANTATION; HYALURONAN; EXPRESSION; OVARIAN; CELLS;

D O I：

10.1016/j.micron.2009.02.005

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The composition of sulfated glycosaminoglycans (GAGs) and the tissue distribution of chondroitin sulfate (CS) were analyzed in deeply infiltrating endometriosis (DIE) of rectosigmoid, using metachromatic staining, and biochemical analysis employing electrophoresis before and after specific enzymatic or chemical degradations, and immunostaining with an antibody against CS. The sulfated GAGs were characterized as dermatan sulfate (DS), heparan sulfate (HS) and CS; and DS strongly predominated compared to HS and CS. Immunostaining procedures showed that CS was concentrated in the endometriosis foci, distributed throughout the stroma around the glands. This is the first report describing the composition of sulfated GAGs and the tissue location of CS in DIE by means of histochemical, biochemical and immunohistochemical analyses. These results confirmed that in DIE of rectosigmoid, as in eutopic endometrium [Nasciutti, L.E., Ferrari, R., Berardo, P.T., Souza, M.L.S., Takiya, C.M., Borojevic, R., Abrao, M.S., Silva, L.C.F., 2006. Distribution of chondroitin sulfate in human endometrium. Micron 37, 544-550], CS was the dominant sulfated GAG in stroma of the lesion foci. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：639 / 645

页数：7

共 25 条

[1] Histological classification of endometriosis as a predictor of response to treatment [J].

Abrao, MS ;

Neme, RM ;

Carvalho, FM ;

Aldrighi, JM ;

Pinotti, JA .

INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS, 2003, 82 (01) :31-40

[2] Pain, mast cells, and nerves in peritoneal, ovarian, and deep infiltrating endometriosis [J].

Anaf, Vincent ;

Chapron, Charles ;

El Nakadi, Issam ;

De Moor, Veronique ;

Simonart, Thierry ;

Noel, Jean-Christophe .

FERTILITY AND STERILITY, 2006, 86 (05) :1336-1343

[3] Laparoscopic excision of posterior vaginal fornix in the treatment of patients with deep endometriosis without rectum involvement: surgical treatment and long-term follow-up [J].

Angioni, S. ;

Peiretti, M. ;

Zirone, M. ;

Palomba, M. ;

Mais, V. ;

Gomel, V. ;

Melis, G. B. .

HUMAN REPRODUCTION, 2006, 21 (06) :1629-1634

[4]

APLIN JD, 1988, CELL TISSUE RES, V253, P231

[5] Presurgical diagnosis of posterior deep infiltrating endometriosis based on a standardized questionnaire [J].

Chapron, C ;

Barakat, H ;

Fritel, X ;

Dubuisson, JB ;

Bréart, G ;

Fauconnier, A .

HUMAN REPRODUCTION, 2005, 20 (02) :507-513

[6] Heparan sulfate-protein interactions: therapeutic potential through structure-function insights [J].

Coombe, DR ;

Kett, WC .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2005, 62 (04) :410-424

[7]

CORNILLIE FJ, 1990, FERTIL STERIL, V53, P978

[8] LOCALIZATION OF HYALURONAN IN REGIONS OF THE HUMAN FEMALE REPRODUCTIVE-TRACT [J].

EDELSTAM, GAB ;

LUNDKVIST, OE ;

WELLS, AF ;

LAURENT, TC .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1991, 39 (08) :1131-1135

[9] KERATAN SULFATE AS A SECRETORY PRODUCT OF HUMAN ENDOMETRIUM - CYCLIC EXPRESSION IN NORMAL WOMEN [J].

GRAHAM, RA ;

LI, TC ;

COOKE, ID ;

APLIN, JD .

HUMAN REPRODUCTION, 1994, 9 (05) :926-930

[10] Fibrosis and smooth muscle metaplasia in rectovaginal endometriosis [J].

Itoga, T ;

Matsumoto, T ;

Takeuchi, H ;

Yamasaki, S ;

Sasahara, N ;

Hoshi, T ;

Kinoshita, K .

PATHOLOGY INTERNATIONAL, 2003, 53 (06) :371-375

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