Rapid method for protein quantitation by Bradford assay after elimination of the interference of polysorbate 80

被引：73

作者：

Cheng, Yongfeng ^{[1
,2
,3
]}

Wei, Haiming ^{[1
,2
,3
,4
]}

Sun, Rui ^{[1
,2
,3
,4
]}

Tian, Zhigang ^{[1
,2
,3
,4
]}

Zheng, Xiaodong ^{[1
,2
,3
]}

机构：

[1] Univ Sci & Technol China, Inst Immunol, Hefei 230027, Anhui, Peoples R China

[2] Univ Sci & Technol China, CAS Key Lab Innate Immun & Chron Dis, Sch Life Sci, Hefei 230027, Anhui, Peoples R China

[3] Univ Sci & Technol China, Med Ctr, Hefei 230027, Anhui, Peoples R China

[4] Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2016年 / 494卷

关键词：

Sucrose; Protein precipitation; Bradford assay; FORMULATION; STABILITY; DETERGENTS;

D O I：

10.1016/j.ab.2015.10.013

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Bradford assay is one of the most common methods for measuring protein concentrations. However, some pharmaceutical excipients, such as detergents, interfere with Bradford assay even at low concentrations. Protein precipitation can be used to overcome sample incompatibility with protein quantitation. But the rate of protein recovery caused by acetone precipitation is only about 70%. In this study, we found that sucrose not only could increase the rate of protein recovery after 1 h acetone precipitation, but also did not interfere with Bradford assay. So we developed a method for rapid protein quantitation in protein drugs even if they contained interfering substances. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：37 / 39

页数：3

共 18 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

PROTEIN PRECIPITATION TECHNIQUES [J].