A recombinant truncated Cry1Ca protein is toxic to lepidopteran insects and forms large cuboidal crystals in insect cells

被引：6

作者：

Aguiar, Raimundo W. S.

Martins, Erica S.

Valicente, Fernando H.

Carneiro, Newton P.

Batista, Andreia C.

Melatti, Viviane M.

Monnerat, Rose G.

Ribeiro, Bergmann M. ^{[1
]}

机构：

[1] Univ Brasilia, Dept Cell Biol, BR-70910900 Brasilia, DF, Brazil

[2] EMBRAPA, Recursos Genet & Biotecnol, BR-70770900 Brasilia, DF, Brazil

[3] EMBRAPA Milho & Sorgo, BR-35701970 Lagoas, MG, Brazil

来源：

CURRENT MICROBIOLOGY | 2006年 / 53卷 / 04期

关键词：

D O I：

10.1007/s00284-005-0502-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A truncated version of the cry1Ca gene from Bacillus thuringiensis was introduced into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) under the control of two promoters. A recombinant virus (vSyncry1c) was isolated and used to infect insect cells in culture and insect larvae. Structural and ultrastructural analysis of insects infected with vSyncry1C showed the formation of large cuboidal crystals inside the cytoplasm of insect cells in culture and in insect cadavers late in infection. Infected insect cell extracts were analyzed by SDS-PAGE and Western blot and showed the presence of a 65-kDa polypeptide probably corresponding to the protease processed form of the toxin. Bioassays using purified recombinant toxin crystals showed a CL50 of 19.49 ng/ml for 2(nd) instar A. gemmatalis larvae and 114.1 ng/ml for S. frugiperda.

引用

页码：287 / 292

页数：6

共 24 条

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