Seasonal variations in detection and transmission of pear decline phytoplasma

被引：20

作者：

Errea, P ^{[1
]}

Aguelo, V ^{[1
]}

Hormaza, JI ^{[1
]}

机构：

[1] SIA DGA, Unidad Fruticultura, Zaragoza, Spain

来源：

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 2002年 / 150卷 / 8-9期

关键词：

DNA amplification; nested-PCR; pear decline; Pyrus communis; specific primers;

D O I：

10.1046/j.1439-0434.2002.00767.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The seasonal variation in the detection of the pear decline phytoplasma was followed in diseased pear trees pertaining to the cultivars 'Limonera', 'Magallon' and 'Monsallard'. Wood and or leaf samples were obtained every two weeks at five different above-ground levels and analyzed using nested-PCR (Polymerase Chain Reaction). The results revealed that it was possible to detect the pathogen during the dormant season in the aerial parts of the diseased trees examined under the environmental conditions used in this study. Moreover, graft transmission experiments showed that the phytoplasmas can be propagated through grafting with phytoplasma-infected dormant buds collected in winter. The results are discussed in terms of their implication for pear cultivation and propagation.

引用

页码：439 / 443

页数：5

共 22 条

[1] DETECTION OF DNA OF PLANT PATHOGENIC MYCOPLASMALIKE ORGANISMS BY A POLYMERASE CHAIN-REACTION THAT AMPLIFIES A SEQUENCE OF THE 16S RNA GENE [J].