The SLGs corresponding to the same S-24-haplotype are perfectly conserved in three different self-incompatible Brassica campestris L

We have identified three strains of the same S-24-haplotype in self-incompatible B. campestris L. Two of them, S-12j and 1-1j, have been derived from Japanese populations and one, 27-1t, from Turkish one. The cDNA clones of SLG(24) (S-24-locus glycoprotein), which linked to the S-locus, were isolated from each strain, and sequenced. Each clone isolated from S-12j, 1-1j and 27-1t, was designated as SLG(24) (S-12j), SLG(24) (1-1j) and SLG(24) (27-1t), respectively. Their nucleotide sequences were completely identical in coding region, 5' non-coding region, and 3' non-coding region, though the position of the polyadenylation site was slightly different among the cDNA clones. This result suggests that the origin of S-24-haplotype in these three strains might be common, and that the nucleotide sequences of SLG(24) of the same S-24-haplotype are completely conserved among different populations. The high conservation of the SLG(24) nucleotide sequences is probably essential for the recognition of self or non-self of self-incompatibility in this S-24-haplotype.