Blowing DNA bubbles

被引：16

作者：

Severin, N.

Zhuang, W.

Ecker, C.

Kalachev, A. A.

Sokolov, I. M.

Rabe, J. P.

机构：

[1] Humboldt Univ, Dept Phys, D-12489 Berlin, Germany

[2] Plasmachem GmbH, D-12489 Berlin, Germany

来源：

NANO LETTERS | 2006年 / 6卷 / 11期

关键词：

D O I：

10.1021/nl061989b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report here experimental observations which indicate that topologically or covalently formed polymer loops embedded in an ultrathin liquid film on a solid substrate can be "blown" into circular "bubbles" during scanning force microscopy (SFM) imaging. In particular, super-coiled vector DNA has been unraveled, moved, stretched, and overstretched to two times its B-form length and then torn apart. We attribute the blowing of the DNA bubbles to the interaction of the tapping SFM tip with the ultrathin liquid film.

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页码：2561 / 2566

页数：6

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