Development and optimization of an analytical method for the determination of Sudan dyes in hot chilli pepper by high-performance liquid chromatography with on-line electrogenerated BrO--luminol chemiluminescence detection

被引:115
作者
Zhang, Yantu
Zhang, Zhujun [1 ]
Sun, Yonghua
机构
[1] Shaanxi Normal Univ, Sch Chem & Mat Sci, Dept Chem, Xian 710062, Peoples R China
[2] Yanan Univ, Dept Chem & Chem Engn, Yanan 716000, Peoples R China
基金
中国国家自然科学基金;
关键词
Sudan dyes; chemiluminescence detector; on-line electrogenerated BrO-; high-performance liquid chromatography; hot chilli pepper;
D O I
10.1016/j.chroma.2006.06.028
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The determination of four Sudan dyes by means of high-performance liquid chromatography (HPLC) with chemiluminescence (CL) detection was proposed. The method was based on the enhancement effect of Sudan dyes on the chemiluminescence reaction between luminol and BrO-, which was on-line electrogenerated by constant current electrolysis. The separation was carried out on Nucleosil RP-C-18 column (250 mm x 4.6 mm i.d., 5 mu m, pore size, 100 angstrom) at 35 degrees C. The mobile phase consisted of a V (methanol): V (0.2% aqueous formic acid) = 90: 10 solution. At a flow-rate of 1.0 ml min(-1), the total run time was 25 min. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. For the four Sudan dyes, the limits of detection (LOD) at a signal-to-noise of 3 ranged from 4 to 8 mu g kg(-1) and the limits of quantification (LOQ) at a signal-to-noise of 10 ranged from 13 to 27 mu g kg(-1). The relative standard deviations (RSD) of intra-and inter-day precision were below 4.4%. The average recoveries for all four Sudan dyes (spiked at the levels of 1.0 and 1.5 mu g kg(-1)) in chilli tomato sauce and hot chilli pepper ranged from 94% to 105%, and the relative standard deviations of the quantitative results were from 2.5 to 4.2%. The proposed method had been successfully applied to the determination of four Sudan dyes in hot chilli products. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:34 / 40
页数:7
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