Characterization and renal protective effect of a polysaccharide from Astragalus membranaceus

被引：100

作者：

Li, Shi-gang ^{[1
]}

Zhang, Yong-qi ^{[1
]}

机构：

[1] Three Gorges Univ, Coll Med, Yichang 443002, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2009年 / 78卷 / 02期

关键词：

Astragalus membranaceus; D-Glucan; Structure; Renal protection; D-GLUCAN; MUSHROOMS; HERBS;

D O I：

10.1016/j.carbpol.2009.04.013

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A water-soluble polysaccharide named as APS was isolated from the roots of Astragalus membranaceus by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its renal protective effect. Its structural characteristics were investigated by FTIR, AMLC, NMR spectroscopy, GLC-MS, methylation analysis, periodate oxidation and Smith degradation. Based on the data obtained, APS was found to be an alpha-(1 -> 4)-D-glucan, with a single alpha-D-glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 3.6 x 10(4) Da. 24 h Urine protein quantification and morphological observation revealed that APS significantly decreased the proteinuria and morphological changes on glomerulonephritis rats induced by cationic Bovine Serum Albumin (C-BSA), indicating APS could have a possible glomerulonephritis therapeutic potential. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：343 / 348

页数：6

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