Gas chromatography-mass spectrometry method for determination of phenylalanine and tyrosine in neonatal blood spots

被引:102
作者
Deng, CH [1 ]
Deng, YH
Wang, B
Yang, XH
机构
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[2] Fudan Univ, Dept Macromol, Shanghai 200433, Peoples R China
[3] Sch Chinese Med, Fuzhou 344100, Jiangxi, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 780卷 / 02期
关键词
phenylalanine; tyrosine;
D O I
10.1016/S1570-0232(02)00632-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper we developed a simple, rapid and sensitive method for the quantitative analysis of phenylalanine (Phe) and tyrosine (Tyr) in dried blood spots of newborns by gas chromatography-mass spectrometry (GC-MS). Phe and Tyr in blood samples were reacted with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide at 120degreesC for 30 min and their corresponding single derivatives were obtained. Phe and Tyr were determined by measurement of their derivatives by GC-MS in the selected ion monitoring mode. Contents of Phe and Tyr in blood spots were calculated by external standard method. The ratio of Phe to Tyr was used for neonatal screening for phenylketonuria. The present method only took a few minutes to perform and required minimal sample preparation. In addition it provided low detection limits of 1.2 mumol l(-1) for Phe and 1.6 mumol l(-1) for Tyr. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:407 / 413
页数:7
相关论文
共 24 条
[1]  
AMIN MR, 1995, J CHROMATOGR B, V663, P201
[2]   CONTENT OF PHENYLALANINE, TYROSINE AND THEIR METABOLITES IN CSF IN PHENYLKETONURIA [J].
ANTOSHECHKIN, AG ;
CHENTSOVA, TV ;
TATUR, VY ;
NARITSIN, DB ;
RAILIAN, GP .
JOURNAL OF INHERITED METABOLIC DISEASE, 1991, 14 (05) :749-754
[3]  
CHACE DH, 1993, CLIN CHEM, V39, P66
[4]   Rapid diagnosis of phenylketonuria and other aminoacidemias by quantitative analysis of amino acids in neonatal blood spots by gas chromatography-mass spectrometry [J].
Deng, CH ;
Shang, CQ ;
Hu, YM ;
Zhang, XM .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 775 (01) :115-120
[5]   Use of the phenylalanine:tyrosine ratio to test newborns for phenylketonuria in a large public health screening programme [J].
Eastman, JW ;
Sherwin, JE ;
Wong, R ;
Liao, CL ;
Currier, RJ ;
Lorey, F ;
Cunningham, G .
JOURNAL OF MEDICAL SCREENING, 2000, 7 (03) :131-135
[6]   Comparison of four different phenylalanine determination methods [J].
Fingerhut, R ;
Stehn, M ;
Kohlschutter, A .
CLINICA CHIMICA ACTA, 1997, 264 (01) :65-73
[7]   Simplified screening for organic acidemia using GC/MS and dried urine filter paper: a study on neonatal mass screening [J].
Fu, XW ;
Iga, M ;
Kimura, M ;
Yamaguchi, S .
EARLY HUMAN DEVELOPMENT, 2000, 58 (01) :41-55
[8]  
GERASIMOVA NS, 1989, CLIN CHEM, V35, P2112
[9]  
GUTHRIE R, 1963, PEDIATRICS, V32, P338
[10]   Gene diagnosis of phenylketonuria by capillary electrophoresis in a novel nongel sieving polymer solution [J].
Han, F ;
Lin, B ;
Xu, Q ;
Shen, Y ;
Wu, G .
CHROMATOGRAPHIA, 1999, 49 (3-4) :179-184