STED microscopy resolves nanoparticle assemblies

被引：93

作者：

Willig, K. I. ^{[1
]}

Keller, J. ^{[1
]}

Bossi, M. ^{[1
]}

Hell, S. W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Nanobiophoton, D-37070 Gottingen, Germany

来源：

NEW JOURNAL OF PHYSICS | 2006年 / 8卷

关键词：

D O I：

10.1088/1367-2630/8/6/106

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We demonstrate the ability of stimulated emission depletion ( STED) microscopy, a far-field fluorescence imaging technique with diffraction-unlimited resolution, to reveal the spatial order of fluorescent nanoparticles. Unlike its confocal counterpart, here STED microscopy resolves the arrangements of densely packed 40 nm beads, supramolecular aggregates in a cell membrane, and colloidal nanoparticles. Both raw and linearly deconvolved data disclose unprecedented details of both biological and non-biological nanopatterns.

引用

页数：8

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