Benzoyl peroxide cytotoxicity evaluated in vitro with the human keratinocyte cell line, RHEK-I

被引:58
作者
Babich, H
Zuckerbraun, HL
Wurzburger, BJ
Rubin, YL
Borenfreund, E
Blau, L
机构
[1] YESHIVA UNIV,STERN COLL WOMEN,DEPT CHEM,NEW YORK,NY 10016
[2] ROCKEFELLER UNIV,LAB ANIM RES CTR,NEW YORK,NY 10021
关键词
benzoyl peroxide; free radicals; keratinocytes; lipid peroxidation; neutral red assay; vitamin E;
D O I
10.1016/0300-483X(95)03189-M
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The human keratinocyte cell line, RHEK-1, was used to evaluate the cytotoxicity of benzoyl peroxide (BZP). As determined with the neutral red (NR) cytotoxicity assay, the 24-h midpoint (NR(50)) toxicity values, in mM, were 0.11 for BZP and 29.5 for benzoic acid, the stable metabolite of BZP. Irreversible cytotoxicity occurred after a I-h exposure to 0.15 mM BZP and greater, When exposed to BZP for 7 days, a lag in growth kinetics was first observed at 0.06 mM BZP, Damage to the integrity of the plasma membrane was evident, as leakage of lactic acid dehydrogenase occurred during a 4-h exposure to BZP at 0.05 mM and greater. Intracellular membranes were also affected, as extensive vacuolization, initially perinuclear but then spreading throughout the cytoplasm, was noted in BZP-stressed cells. The generation of reactive free radicals from BZP was suggested by the following: the intracellular content of glutathione was lowered in cells exposed to BZP; cells pretreated with the glutathione-depleting agent, chlorodinitrobenzene, were hypersensitive to a subsequent challenge with BZP; lipid peroxidation by BZP was inducible in the presence of Fe2+; and cells previously maintained in a medium amended with vitamin E, an antioxidant, were more resistant to BZP, showed less lipid peroxidation in the presence of BZP + Fe2+ and did not develop the extensive intracellular vacuolization as compared to non-vitamin E maintained cells.
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页码:187 / 196
页数:10
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