Comparison of several methods to determine methicillin-resistance in Staphylococcus aureus with focus on borderline strains

被引：10

作者：

Nicola, F ^{[1
]}

Bantar, C ^{[1
]}

Canigia, LF ^{[1
]}

Relloso, S ^{[1
]}

Bianchini, H ^{[1
]}

Smayevsky, J ^{[1
]}

机构：

[1] Ctr Educ Med & Invest Clin, Dept Microbiol, RA-1425 Buenos Aires, DF, Argentina

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2000年 / 36卷 / 02期

关键词：

D O I：

10.1016/S0732-8893(99)00144-3

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We compared the performance of several phenotypic tests to detect methicillin-resistant Staphylococcus aureus, with special focus on borderline strains. The reliability of the agar screen oxacillin and BBL Crystal tests was asserted for all methicillin-susceptible (n = 25), resistant (n = 29) and borderline beta-lactamase-hyperproducer (n = 10) strains. Whereas these tests failed to detect 4 of 5 rare borderline strains containing few cells with high-level methicillin resistance (i.e., a frequency of 10(-7)-10(-8)), a "two-temperature" disk diffusion method, performed simultaneously at 35 and 42 degrees C, detected all of such strains. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：91 / 93

页数：3

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