Characterization and functional role of leptin receptor in bovine adrenal medullary cells

被引：31

作者：

Yanagihara, N

Utsunomiya, K

Cheah, TB

Hirano, H

Kajiwara, K

Hara, K

Nakamura, E

Toyohira, Y

Uezono, Y

Ueno, S

Izumi, F

机构：

[1] Univ Occupat & Environm Hlth, Sch Med, Dept Pharmacol, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

[2] Univ Occupat & Environm Hlth, Sch Med, Dept Biochem, Kitakyushu, Fukuoka 8078555, Japan

[3] Univ Newcastle, Fac Med & Hlth Sci, Discipline Med Biochem, Newcastle, NSW 2308, Australia

来源：

BIOCHEMICAL PHARMACOLOGY | 2000年 / 59卷 / 09期

关键词：

bovine adrenal medulla; catecholamine synthesis; I-125-leptin binding; leptin receptor;

D O I：

10.1016/S0006-2952(00)00240-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We report here the characterization and functional roles of the leptin receptor (ObR) in bovine adrenal medullary cells. The plasma membranes isolated from bovine adrenal medulla showed a single class of specific binding sites of I-125-leptin with an apparent K-d Of 6.6 nM and B-max of 62 fmol/mg protein. ObRa but not ObRb mRNA was detected in bovine adrenal medulla by reverse transcriptase-polymerase chain reaction. Incubation of cultured adrenal medullary cells with leptin (3-30 nM) for 20 min resulted in a significant increase in [C-14]catecholamine synthesis from [C-14]tyrosine without any change in catecholamine secretion. These findings suggest that leptin stimulates catecholamine synthesis through its receptors in bovine adrenal medullary cells. (C) 2000 Elsevier Science Inc.

引用

页码：1141 / 1145

页数：5

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