Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA

被引：114

作者：

Saitoh, S

Chabes, A

McDonald, WH

Thelander, L

Yates, JR

Russell, P

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[3] Umea Univ, Dept Med Biochem & Biophys, SE-90187 Umea, Sweden

来源：

CELL | 2002年 / 109卷 / 05期

关键词：

D O I：

10.1016/S0092-8674(02)00753-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fission yeast Cid13 and budding yeast Trf4/5 are members of a newly identified nucleotidyltransferase family conserved from yeast to man. Trf4/5 are thought to be essential DNA polymerases. We report that Cid13 is a poly(A) polymerase. Unlike conventional poly(A) polymerases, which act in the nucleus and indiscriminately polyadenylate, all mRNA, Cid13 is a cytoplasmic enzyme that specifically targets suc22 mRNA that encodes a subunit of ribonucleotide reductase (RNR). cid13 mutants have reduced dNTP pools and are sensitive to hydroxyurea, an RNR inhibitor. We propose that Cid13 defines a cytoplasmic form of poly(A) polymerase important for DNA replication and genome maintenance.

引用

页码：563 / 573

页数：11

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[1] HIGHER-ORDER CHROMOSOME STRUCTURE IS AFFECTED BY COLD-SENSITIVE MUTATIONS IN A SCHIZOSACCHAROMYCES-POMBE GENE CRM1+ WHICH ENCODES A 115-KD PROTEIN PREFERENTIALLY LOCALIZED IN THE NUCLEUS AND AT ITS PERIPHERY [J].