A thermostable vacuolar-type membrane pyrophosphatase from the archaeon Pyrobaculum aerophilum:: implications for the origins of pyrophosphate-energized pumps

被引:59
作者
Drozdowicz, YM
Lu, YP
Patel, V
Fitz-Gibbon, S
Miller, JH
Rea, PA [1 ]
机构
[1] Univ Penn, Dept Biol, Inst Plant Sci, Philadelphia, PA 19104 USA
[2] Univ Calif Los Angeles, Dept Microbiol & Mol Genet, Los Angeles, CA 90095 USA
基金
美国国家科学基金会;
关键词
archaeon; inorganic pyrophosphatase; proton pump; pyrophosphate; vacuolar-type H+-pyrophosphatase; Pyrobaculum aerophilum;
D O I
10.1016/S0014-5793(99)01404-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Vacuolar-type H+-translocating pyrophosphatases (V-PPases) hale been considered to be restricted to plants, a few species of phototrophic proteobacteria and protists. Here, we describe PVP, a thermostable, sequence-divergent V-PPase from the facultatively aerobic hyperthermophilic archaeon Pyrobaculum nel aerophilum. PVP shares only 38% sequence identity with both the prototypical V-PPase from Arabidopsis thaliana and the H+-PPi synthase from Rhodospirillum rubrum, yet possesses most of the structural features characteristic of V-PPases, Heterologous expression of PVP in Saccharomyces cerevisiae yields a M-r 64 000 membrane polypeptide that specifically catalyzes Mg2+-dependent PPi hydrolysis. The existence of PVP implies that PPi-energized H+-translocation is phylogenetically more deeply rooted than previously: thought, (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:505 / 512
页数:8
相关论文
共 34 条
[1]  
Baltscheffsky Herrick, 1996, P1
[2]   A pyrophosphate synthase gene:: Molecular cloning and sequencing of the cDNA encoding the inorganic pyrophosphate synthase from Rhodospirillum rubrum [J].
Baltscheffsky, M ;
Nadanaciva, S ;
Schultz, A .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1364 (03) :301-306
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii [J].
Bult, CJ ;
White, O ;
Olsen, GJ ;
Zhou, LX ;
Fleischmann, RD ;
Sutton, GG ;
Blake, JA ;
FitzGerald, LM ;
Clayton, RA ;
Gocayne, JD ;
Kerlavage, AR ;
Dougherty, BA ;
Tomb, JF ;
Adams, MD ;
Reich, CI ;
Overbeek, R ;
Kirkness, EF ;
Weinstock, KG ;
Merrick, JM ;
Glodek, A ;
Scott, JL ;
Geoghagen, NSM ;
Weidman, JF ;
Fuhrmann, JL ;
Nguyen, D ;
Utterback, TR ;
Kelley, JM ;
Peterson, JD ;
Sadow, PW ;
Hanna, MC ;
Cotton, MD ;
Roberts, KM ;
Hurst, MA ;
Kaine, BP ;
Borodovsky, M ;
Klenk, HP ;
Fraser, CM ;
Smith, HO ;
Woese, CR ;
Venter, JC .
SCIENCE, 1996, 273 (5278) :1058-1073
[5]  
CLAROS MG, 1994, COMPUT APPL BIOSCI, V10, P685
[6]   EVOLUTIONARY CONSERVATION OF THE ACTIVE-SITE OF SOLUBLE INORGANIC PYROPHOSPHATASE [J].
COOPERMAN, BS ;
BAYKOV, AA ;
LAHTI, R .
TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (07) :262-266
[7]   Reverse gyrase from the hyperthermophilic bacterium Thermotoga maritima:: Properties and gene structure [J].
De la Tour, CB ;
Portemer, C ;
Kaltoum, H ;
Duguet, M .
JOURNAL OF BACTERIOLOGY, 1998, 180 (02) :274-281
[8]  
Driessen AJM, 1996, FEMS MICROBIOL REV, V18, P139
[9]   A fosmid-based genomic map and identification of 474 genes of the hyperthermophilic archaeon Pyrobaculum aerophilum [J].
FitzGibbon, S ;
Choi, AJ ;
Miller, JH ;
Stetter, KO ;
Simon, MI ;
Swanson, R ;
Kim, UJ .
EXTREMOPHILES, 1997, 1 (01) :36-51
[10]  
García-Donas I, 1998, PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V, P3679