Effect of the preovulatory gonadotropin surge on matrix metalloproteinase (MMP)-14, MMP-2, and tissue inhibitor of metalloproteinases-2 expression within bovine periovulatory follicular and luteal tissue

被引:35
作者
Bakke, LJ
Dow, M
Cassar, CA
Peters, MW
Pursley, JR
Smith, GW
机构
[1] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Physiol, E Lansing, MI 48824 USA
关键词
follicle; granulosa cells; ovary; ovulation; theca cells;
D O I
10.1095/biolreprod66.6.1627
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The matrix metalloproteinases (MMPs) have been implicated in the ovulatory process, but the specific roles of individual MMPs are unclear. This study examined the effect of the preovulatory gonadotropin surge on localization and regulation of MMP-2, MMP-14, and tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA and MMP-2 and TIMP-2 activity in bovine preovulatory follicles and new corpora lutea (CL). Ovaries containing ovulatory follicles or new CL were collected at approximately 0, 6, 12, 18, 24, and 48 h (CL) after a GnRH-induced gonadotropin surge. Messenger RNA for TIMP-2 and MMP-14 increased within 6 and 24 h of the gonadotropin surge, respectively, whereas MMP-2 mRNA was constitutively expressed. Activity for MMP-2 in follicular fluid and follicle homogenates was not changed, but follicular fluid TIMP-2 activity increased in response to the gonadotropin surge. Messenger RNA for MMP-2 was localized to the thecal layer of bovine preovulatory follicles, whereas MMP-14 mRNA was localized primarily to the thecal layer and adjacent ovarian stroma. Expression of MMP-14 was also observed in the granulosal layer after the gonadotropin surge. In contrast, TIMP-2 mRNA was localized predominantly to the granulosal layer with intense expression in the antral portion of the granulosal layer in response to the gonadotropin surge. These data support the hypothesis that increased expression of MMP-14 and TIMP-2 may help regulate follicle rupture and/or the ovulatory follicle-CL transition in cattle.
引用
收藏
页码:1627 / 1634
页数:8
相关论文
共 38 条
[1]   Proteinases and extracellular matrix remodeling [J].
Alexander, C. M. ;
Werb, Z. .
CURRENT OPINION IN CELL BIOLOGY, 1989, 1 (05) :974-982
[2]   DISTRIBUTION OF BINDING-SITES FOR HUMAN CHORIONIC-GONADOTROPIN IN PREOVULATORY FOLLICLE OF RAT [J].
AMSTERDAM, A ;
KOCH, Y ;
LIEBERMAN, ME ;
LINDNER, HR .
JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :894-900
[3]   Intermolecular autolytic cleavage can contribute to the activation of progelatinase A by cell membranes [J].
Atkinson, SJ ;
Crabbe, T ;
Cowell, S ;
Ward, RV ;
Butler, MJ ;
Sato, H ;
Seiki, M ;
Reynolds, JJ ;
Murphy, G .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (51) :30479-30485
[4]  
BRANNSTROM M, 1988, ENDOCRINOLOGY, V122, P1715
[5]   INHIBITION OF OVULATION IN THE PERFUSED RAT OVARY BY THE SYNTHETIC COLLAGENASE INHIBITOR-SC-44463 [J].
BUTLER, TA ;
ZHU, C ;
MUELLER, RA ;
FULLER, GC ;
LEMAIRE, WJ ;
WOESSNER, JF .
BIOLOGY OF REPRODUCTION, 1991, 44 (06) :1183-1188
[6]   Expression of matrix metalloproteinases and their tissue inhibitor messenger ribonucleic acids in macaque periovulatory granulosa cells: Time course and steroid regulation [J].
Chaffin, CL ;
Stouffer, RL .
BIOLOGY OF REPRODUCTION, 1999, 61 (01) :14-21
[7]   Collagenase and gelatinase messenger ribonucleic acid expression and activity during follicular development in the rat ovary [J].
Cooke, RG ;
Nothnick, WB ;
Komar, C ;
Burns, P ;
Curry, TE .
BIOLOGY OF REPRODUCTION, 1999, 61 (05) :1309-1316
[8]   Cyclic changes in the matrix metalloproteinase system in the ovary and uterus [J].
Curry, TE ;
Osteen, KG .
BIOLOGY OF REPRODUCTION, 2001, 64 (05) :1285-1296
[9]  
Curry TE, 2000, TISSUE INHIBITORS OF METALLOPROTEINASES IN DEVELOPMENT AND DISEASE, PROCEEDINGS, P119
[10]  
ESPEY LL, 1994, PHYSL REPROD, V1, P725