EXAFS spectroscopic evidence for an Fe=O unit in the Fe(IV) intermediate observed during oxygen activation by taurine:α-ketoglutarate dioxygenase

被引：267

作者：

Riggs-Gelasco, PJ ^{[1
]}

Price, JC

Guyer, RB

Brehm, JH

Barr, EW

Bollinger, JM

Krebs, C

机构：

[1] Coll Charleston, Dept Chem & Biochem, Charleston, SC 29424 USA

[2] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

[3] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 26期

关键词：

D O I：

10.1021/ja048255q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The Fe(II)- and α-ketoglutarate-dependent dioxygenases catalyze hydroxylation reactions of considerable biomedical and environmental significance. Recently, the first oxidized iron intermediate in the reaction of a member of this family, taurine:α-ketoglutarate dioxygenase (TauD), was detected and shown to be a high-spin Fe(IV) complex. In this study we have used X-ray absorption spectroscopy to demonstrate the presence of a short (1.62 Å) interaction between the iron and one of its ligands in the Fe(IV) intermediate but not in the Fe(II) starting complex. The detection of this interaction strongly corroborates the hypothesis that the intermediate contains an Fe=O structural motif. Copyright © 2004 American Chemical Society.

引用

页码：8108 / 8109

页数：2

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