Simple and efficient vitrification procedure for cryopreservation of mouse embryos

被引：86

作者：

Nakao, K ^{[1
]}

Nakagata, N ^{[1
]}

Katsuki, M ^{[1
]}

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT DNA BIOL & EMBRYO ENGN,MINATO KU,TOKYO 108,JAPAN

来源：

EXPERIMENTAL ANIMALS | 1997年 / 46卷 / 03期

关键词：

cryopreservation; mouse embryo; simple vitrification;

D O I：

10.1538/expanim.46.231

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Mouse pronuclear oocytes and 2-cell embryos derived from in vitro fertilization were cryopreserved by a novel simple vitrification procedure. Most cryopreserved oocytes/embryos were morphologically normal after warming, and 89-92% of them developed to the blastocyst stage during the culture. Moreover, the rate of morphologically normal pronuclear oocytes after being repeatedly cooled and warmed three times was as high as that of oocytes cooled and warmed only once, and 85% of them developed to the blastocyst stage. In addition, 43-57% of the cryopreserved oocytes/embryos transferred to recipients had developed normally to live fetuses observed on day 18.5 of pregnancy.

引用

页码：231 / 234

页数：4

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