Gene structure of human cholecystokinin (CCK) type-A receptor: body fat content is related to CCK type-A receptor gene promoter polymorphism

被引：67

作者：

Funakoshi, A

Miyasaka, K

Matsumoto, H

Yamamori, S

Takiguchi, S

Kataoka, K

Takata, Y

Matsusue, K

Kono, A

Shimokata, H

机构：

[1] Kyushu Natl Canc Ctr, Dept Gastroenterol, Minami Ku, Fukuoka 8111395, Japan

[2] Tokyo Metropolitan Inst Gerontol, Dept Clin Physiol, Tokyo 1730015, Japan

[3] INC, Mitsubishi Kagaku Bioclin Labs, Dept Cellular Genet, Tokyo 1748555, Japan

[4] Kyushu Natl Canc Ctr, Dept Chemotherapy, Fukuoka 8111395, Japan

[5] Natl Inst Longev Sci, Dept Epidemiol, Ohbu 4748522, Japan

来源：

FEBS LETTERS | 2000年 / 466卷 / 2-3期

关键词：

fat deposition; CCK-A receptor promoter; capsite hunting; polymorphism; leptin;

D O I：

10.1016/S0014-5793(00)01080-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The transcriptional start site of the human cholecystokinin (CCK)-A receptor gene was determined by the Capsite Hunting method. Two sequence changes were detected, a G to T change in nucleotide -128, and an A to G change in nucleotide -81, The homozygote (T/T, G/G) was detected in 25 of 1296 individuals (1.9%) in the cohort study. This polymorphism showed a significantly higher percent body fat and higher levels of serum insulin and leptin, compared with wild type and heterozygotes. Our study provided the possibility that polymorphism in the promoter region of the CCK-A receptor gene may be one of genetic factors affecting fat deposition. (C) 2000 Federation of European Biochemical Societies.

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页码：264 / 266

页数：3

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