Vitamin A differentially regulates RANKL and OPG expression in human osteoblasts

被引：38

作者：

Jacobson, A ^{[1
]}

Johansson, S ^{[1
]}

Branting, M ^{[1
]}

Melhus, H ^{[1
]}

机构：

[1] Univ Uppsala Hosp, Dept Med Sci, Uppsala, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 322卷 / 01期

关键词：

vitamin A; osteoprotegerin; bone resorption retinoic acid; osteoporosis; receptor for activation of NF-kappa B ligand; retinoic acid receptor; RAR-antagonist; osteoblast; bone;

D O I：

10.1016/j.bbrc.2004.07.092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

All-trans-retinoic acid (ATRA) induces bone resorption, but the molecular mechanisms are unknown. We have studied the effect of ATRA on osteoprotegerin (OPG) and receptor activator of NF-kappaB ligand (RANKL) expression in human MG-63 osteosarcoma cells and primary osteoblast-like cultures. ATRA dose-dependently down-regulated protein levels of OPG in MG-63 cells, with a maximum (-56%) observed at a dose of 10(-6) M. This effect was confirmed with quantitative real-time PCR, where OPG mRNA was decreased after 4 h (-68%) in primary cultures and after 8 h (-87%) in MG-63 cells. The reduction in OPG expression was inhibited by a retinoic acid receptor (RAR)-antagonist and was mimicked by a RARbeta, gamma-agonist, indicating that the ATRA effect is mediated by these receptors. In primary cultures we found a threefold induction of RANKL mRNA expression. Thus, the RANKL/OPG ratio was markedly increased, suggesting a potential mechanism of ATRA-induced bone resorption. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：162 / 167

页数：6

共 45 条

[1] A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function [J].