Isolation and characterisation of collagens from the skin of largefin longbarbel catfish (Mystus macropterus)

被引：188

作者：

Zhang, Min ^{[1
]}

Liu, Wentao ^{[1
]}

Li, Guoying ^{[1
]}

机构：

[1] Sichuan Univ, Natl Engn Lab Clean Technol Leather Mfg, Chengdu 610065, Peoples R China

来源：

FOOD CHEMISTRY | 2009年 / 115卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Collagen; Catfish; Mystus macropterus; Skin; Characterisation; ACID-SOLUBLE COLLAGEN; BIOCHEMICAL-PROPERTIES; BLACK DRUM; FISH; BONE;

D O I：

10.1016/j.foodchem.2009.01.006

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from the skin of largefin longbarbel catfish (Mystus macropterus) with yields of 16.8% and 28.0%, respectively, on the basis of dry weight. Both ASC and PSC contained alpha 1 and alpha 2 chains and the amino acid composition of collagen was close to that of calf skin type I collagen. The intrinsic viscosities of ASC and PSC were 14.9 dl/g and 14.5 dl/g, respectively. Similar ultraviolet and FTIR spectra of ASC and PSC were observed. However, peptide maps of ASC and PSC, hydrolysed by trypsin, revealed some differences in primary structures between the two fractions. Denaturation temperatures of ASC and PSC were 32.1 degrees C and 31.6 degrees C. respectively. The higher T-m showed that it is possible to use largefin longbarbel catfish skin collagen as an alternative source of vertebrate collagens for industrial purposes. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：826 / 831

页数：6

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