Solid-medium-integrated impedimetric biosensor for real-time monitoring of microorganisms

被引:19
作者
Choi, Ahmi [2 ]
Park, Jae-Sung [1 ]
Jung, Hyo-Il [1 ,2 ]
机构
[1] Yonsei Univ, Sch Mech Engn, Seoul 120749, South Korea
[2] Yonsei Univ, Natl Core Res Ctr Nanomed Technol, Seoul 120749, South Korea
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 137卷 / 01期
关键词
Solid medium; Impedance microbiology; Biosensor; Real-time monitoring; ESCHERICHIA-COLI O157-H7; RAPID DETECTION; IMPEDANCE BIOSENSOR; CONDUCTIMETRY; IMMUNOSENSOR; COMBINATION; LISTERIA; GROWTH; SENSOR;
D O I
10.1016/j.snb.2008.12.062
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Rapid, real-time detection of pathogenic microorganisms is an emerging and quickly evolving field of research, especially with regard to microorganisms that pose a major threat to public health. Herein, a new method that uses bioimpedance and solid culture medium for the real-time monitoring of bacterial growth is introduced. We fabricated a new impedimetric biosensor by integrating solid medium and two plane electrodes attached on two facing sides of an acryl well, during bioelectrical impedance analysis, the solid medium showed the characteristics of a homogenous conductive material. In a real-time impedance measurement, our solid medium biosensor could monitor bacterial growth in situ with a detection time of similar to 4 h. We applied different culture media (e.g. Tryptic Soy Agar, Luria Agar, and Nutrient Agar) to our biosensor and found that a low conductive medium was optimal for the monitoring of the bacterial growth due to low threshold in the variation of the impedance signal. Our data indicate that the solid medium biosensor is useful for detection of pathogenic microorganisms, thereby providing a new analytical tool for impedance microbiology. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:357 / 362
页数:6
相关论文
共 31 条
[1]   A POROUS AGAR MEDIUM FOR IMPROVING THE GROWTH OF PLANTS UNDER STERILE CONDITIONS [J].
BARRETTLENNARD, EG ;
DRACUP, M .
PLANT AND SOIL, 1988, 108 (02) :294-298
[2]   Rapid detection of fungal α-amylase in the work environment with a lateral flow immunoassay [J].
Bogdanovic, Jelena ;
Koets, Marjo ;
Sander, Ingrid ;
Wouters, Inge ;
Meijster, Tim ;
Heederik, Dick ;
van Amerongen, Aart ;
Doekes, Gert .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2006, 118 (05) :1157-1163
[3]   AN INVESTIGATION OF INDIRECT CONDUCTIMETRY FOR DETECTION OF SOME FOOD-BORNE BACTERIA [J].
BOLTON, FJ .
JOURNAL OF APPLIED BACTERIOLOGY, 1990, 69 (05) :655-661
[4]   A METHOD AND MEDIUM FOR THE ELECTRICAL DETECTION OF LISTERIA SPP FROM FOOD [J].
CAPELL, CJ ;
KIRBY, RM ;
MOSS, MO .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1995, 25 (02) :169-177
[5]   Rapid estimation of Escherichia coli in live marine bivalve shellfish using automated conductance measurement [J].
Dupont, J ;
Menard, D ;
Herve, C ;
Chevalier, F ;
Beliaeff, B ;
Minier, B .
JOURNAL OF APPLIED BACTERIOLOGY, 1996, 80 (01) :81-90
[6]  
Eden R., 1984, IMPEDANCE MICROBIOLO
[7]   Airborne infectious disease and the suppression of pulmonary bioaerosols [J].
Fiegel, J ;
Clarke, R ;
Edwards, DA .
DRUG DISCOVERY TODAY, 2006, 11 (1-2) :51-57
[8]   Self-assembled monolayers-based immunosensor for detection of Escherichia coli using electrochemical impedance spectroscopy [J].
Geng, Ping ;
Zhang, Xinai ;
Meng, Weiwei ;
Wang, Qingjiang ;
Zhang, Wen ;
Jin, Litong ;
Feng, Zhen ;
Wu, Zirong .
ELECTROCHIMICA ACTA, 2008, 53 (14) :4663-4668
[9]  
Gómez R, 2002, MATER RES SOC SYMP P, V729, P161
[10]   Microfluidic Biochip for Impedance Spectroscopy of Biological Species [J].
Gomez, R. ;
Bashir, R. ;
Sarikaya, A. ;
Ladisch, M. R. ;
Sturgis, J. ;
Robinson, J. P. ;
Geng, T. ;
Bhunia, A. K. ;
Apple, H. L. ;
Wereley, S. .
BIOMEDICAL MICRODEVICES, 2001, 3 (03) :201-209