Archived or directly swabbed latent fingerprints as a DNA source for STR typing

被引:40
作者
Schulz, MM [1 ]
Reichert, W [1 ]
机构
[1] Heidelberg Univ, Inst Legal Med, D-69115 Heidelberg, Germany
关键词
DNA typing; fingerprint processing; epithelial cells; forensic science; short tandem repeat;
D O I
10.1016/S0379-0738(02)00092-0
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
Former studies [Nature (1997) 387, Electrophoresis 20 (1999) 2870, P. Van Renterghem, D. Leonard, C. De Greef, Progress in Forensic Genetics, Vol. 8, 2000, p. 501 J. Forensic Sci. 45 (3) (2000) 687] have shown that even a single skin contact, documented by a latent fingerprint, can transfer enough DNA for a genetic analysis. It was proven in these studies that it is possible to swab fingerprints from surfaces [Nature (1997) 387, Electrophoresis 20 (1999) 2870, P. Van Renterghem, D. Leonard, C. De Greef, Progress in Forensic Genetics, Vol. 8, 2000, p. 501] and use them as a DNA source. Usually, however, discovered fingerprints are removed with scotch tape and placed on evidence cards for further investigation. In this study, we tried to assess the potential use of latent fingerprints as a DNA source for STR typing. The materials (magnetic powder, soot powder and scotch tape) used for visualization and archiving fingerprints in Germany were tested for their PCR inhibitory characteristics. Then, fingerprints were placed on clean glass surfaces, visualized and tested for their usefulness as a DNA source. Obtained DNA was quantified and tested in an STR system. Partly it proved possible to type fingerprints taken directly from the surface as well as fingerprints removed from the surface with scotch tape. (C) 2002 Published by Elsevier Science Ireland Ltd.
引用
收藏
页码:128 / 130
页数:3
相关论文
共 8 条
[1]   Structural variation in the alleles of a short tandem repeat system at the human alpha fibrinogen locus [J].
Barber, MD ;
McKeown, BJ ;
Parkin, BH .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 1996, 108 (04) :180-185
[2]   DNA fingerprinting from single cells [J].
Findlay, I ;
Taylor, A ;
Quirke, P ;
Frazier, R ;
Urquhart, A .
NATURE, 1997, 389 (6651) :555-556
[3]   An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA [J].
Gill, P ;
Whitaker, J ;
Flaxman, C ;
Brown, N ;
Buckleton, J .
FORENSIC SCIENCE INTERNATIONAL, 2000, 112 (01) :17-40
[4]  
Van Hoofstat DEO, 1999, ELECTROPHORESIS, V20, P2870, DOI 10.1002/(SICI)1522-2683(19991001)20:14<2870::AID-ELPS2870>3.0.CO
[5]  
2-V
[6]  
Van Renterghem P, 2000, INT CONGR SER, P501
[7]  
VANOORSCHOT R, 1997, NATURE, P387
[8]  
Zamir A, 2000, J FORENSIC SCI, V45, P687