Freeze-fracture electron microscopic and calorimetric studies on microscopic states of surface-modified liposomes with poly(ethylene glycol) chains

被引：18

作者：

Hashizaki, K

Itoh, C

Sakai, H

Yokoyama, S

Taguchi, H

Saito, Y

Ogawa, N

Abe, M

机构：

[1] Sci Univ Tokyo, Fac Sci & Technol, Noda, Chiba 2788510, Japan

[2] Nihon Univ, Coll Pharm, Funabashi, Chiba 2748555, Japan

[3] NOF Co, Oleo Chem Res Lab, Kawasaki Ku, Kawasaki, Kanagawa 2100865, Japan

[4] Sci Univ Tokyo, Inst Colloid & Interface Sci, Shinjuku Ku, Tokyo 1620825, Japan

[5] Kyoritsu Coll Pharm, Minato Ku, Tokyo 1058512, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2000年 / 17卷 / 04期

关键词：

liposomes; phospholipid with poly (ethylene glycol) chain; freeze-fracture electron microscopy; phase transition temperature; differential scanning calorimetry;

D O I：

10.1016/S0927-7765(99)00114-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The differential scanning calorimetry (DSC) and the freeze-fracture electron microscopy of dipalmitoyl phosphatidylcholine (DPPC) liposomes containing distearoyl-N-monomethoxy poly(ethylene glycol)-succinyl-phosphatidylethanolamines (PEG-DSPE) were carried out. The DSC peak of DPPC liposomes containing PEG-DSPE had a shoulder. The main phase transition temperature of DPPC bilayer membranes containing PEG-DSPE whose molecular weight of PEG is less than 3000 was slightly shifted to a higher temperature, while that containing PEG-DSPE whose molecular weight of PEG is more than 5000 was slightly shifted to a lower temperature. The electron micrographs of freeze-fracture replicas of DPPC liposomes containing PEG-DSPE quenched from 37 +/- 2 degrees C exhibited banded and planar textures, suggesting the lateral phase separation in the bilayer membranes. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：275 / 282

页数：8

共 24 条

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