A crucial role for the putative Arabidopsis topoisomerase VI in plant growth and development

被引:103
作者
Yin, YH
Cheong, H
Friedrichsen, D
Zhao, YD
Hu, JP
Mora-Garcia, S
Chory, J [1 ]
机构
[1] Salk Inst Biol Studies, Howard Hughes Med Inst, La Jolla, CA 92037 USA
[2] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA
关键词
D O I
10.1073/pnas.152337599
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plant steroid hormones, brassinosteroids (BRs), play important roles throughout plant growth and development. Plants defective in BR biosynthesis or perception display cell elongation defects and severe dwarfism. Two dwarf mutants named bin3 and bin5 with identical phenotypes to each other display some characteristics of BR mutants and are partially insensitive to exogenously applied BRs. In the dark, bin3 or bin5 seedlings are de-etiolated with short hypocotyls and open cotyledons. Light-grown mutant plants are dwarfs with short petioles, epinastic leaves, short inflorescence stems, and reduced apical dominance. We cloned BIN3 and BIN5 and show that BIN5 is one of three putative Arabidopsis SPO11 homologs (AtSPO11-3) that also shares significant homology to archaebacterial topoisomerase VI (TOP6) subunit A, whereas BIN3 represents a putative eukaryotic homolog of TOP6B. The pleiotropic dwarf phenotypes of bin5 establish that, unlike all of the other SPO11 homologs that are involved in meiosis, BIN5/AtSPO11-3 plays a major role during somatic development. Furthermore, microarray analysis of the expression of about 5500 genes in bin3 or bin5 mutants indicates that about 321 genes are down-regulated in both of the mutants, including 18 of 30 BR-induced genes. These results suggest that BIN3 and BIN5 may constitute an Arabidopsis topoisomerase VI that modulates expression of many genes, including those regulated by BRs.
引用
收藏
页码:10191 / 10196
页数:6
相关论文
共 42 条
[1]   Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11 [J].
Baudat, F ;
Manova, K ;
Yuen, JP ;
Jasin, M ;
Keeney, S .
MOLECULAR CELL, 2000, 6 (05) :989-998
[2]   An atypical topoisomerase II from archaea with implications for meiotic recombination [J].
Bergerat, A ;
deMassy, B ;
Gadelle, D ;
Varoutas, PC ;
Nicolas, A ;
Forterre, P .
NATURE, 1997, 386 (6623) :414-417
[3]   Multiple roles of Spo11 in meiotic chromosome behavior [J].
Celerin, M ;
Merino, ST ;
Stone, JE ;
Menzie, AM ;
Zolan, ME .
EMBO JOURNAL, 2000, 19 (11) :2739-2750
[4]   ARABIDOPSIS THALIANA MUTANT THAT DEVELOPS AS A LIGHT-GROWN PLANT IN THE ABSENCE OF LIGHT [J].
CHORY, J ;
PETO, C ;
FEINBAUM, R ;
PRATT, L ;
AUSUBEL, F .
CELL, 1989, 58 (05) :991-999
[5]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[6]   A brassinosteroid-insensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development [J].
Clouse, SD ;
Langford, M ;
McMorris, TC .
PLANT PHYSIOLOGY, 1996, 111 (03) :671-678
[7]   Molecular genetic studies confirm the role of brassinosteroids in plant growth and development [J].
Clouse, SD .
PLANT JOURNAL, 1996, 10 (01) :1-8
[8]   Brassinosteroids: Essential regulators of plant growth and development [J].
Clouse, SD ;
Sasse, JM .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :427-451
[9]   The molecular basis of plant cell wall extension [J].
Darley, CP ;
Forrester, AM ;
McQueen-Mason, SJ .
PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) :179-195
[10]   Meiotic recombination in C-elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis [J].
Dernburg, AF ;
McDonald, K ;
Moulder, G ;
Barstead, R ;
Dresser, M ;
Villeneuve, AM .
CELL, 1998, 94 (03) :387-398