Positive role of peptidoglycan breaks in lactococcal biofilm formation

被引:72
作者
Mercier, C
Durrieu, C
Briandet, R
Domakova, E
Tremblay, J
Buist, G
Kulakauskas, S
机构
[1] INRA, Unite Rech Laitieres & Genet Appl, F-78352 Jouy En Josas, France
[2] INRA, Unite Rech Bioadhes & Hyg Mat, F-91744 Massy, France
[3] Univ Groningen, Dept Genet, Groningen Biomol Sci & Biotechnol Inst, NL-9751 NN Haren, Netherlands
关键词
D O I
10.1046/j.1365-2958.2002.03160.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacterial attachment to solid matrices depends on adhesive molecules present on the cell surface. Here we establish a positive correlation between peptidoglycan (PG) breaks, rather than particular molecules, and biofilm-forming capacity in the Gram-positive bacterium Lactococcus lactis. The L. lactis acmA strain, which is defective in PG hydrolase, adhered less efficiently than the wild-type (wt) strain to different solid surfaces and was unable to form biofilms. These phenotypes were abolished by addition of lysozyme, a PG hydrolytic enzyme. Thus, the presence of PG breaks introduced by PG hydrolase, and not the AcmA protein itself, appears to be responsible for biofilm formation. Two different genetic screens confirmed the importance of PG breaks in L. lactis biofilm formation. Using the chain-forming ability of the acmA strain as a phenotypic indicator of PG integrity, we selected for insertional mutants generating short chains. Five independent mutants were all mapped to ponA, which encodes the PG synthesis enzyme PBP1A. Double acmA ponA mutants displayed increased adhesion and biofilm-forming capacity. Direct selection for strains with increased biofilm-forming capacity resulted in the isolation of another five mutations in ponA. Based on these results, we conclude that PG breaks are important for both adhesion and biofilm formation in L. lactis.
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页码:235 / 243
页数:9
相关论文
共 32 条
[1]  
Amann R. I., MOL MICROBIAL ECOLOG, P1
[2]  
[Anonymous], NEW COMPREHENSIVE BI
[3]   Interactions between biofilms and the environment [J].
Beveridge, TJ ;
Makin, SA ;
Kadurugamuwa, JL ;
Li, ZS .
FEMS MICROBIOLOGY REVIEWS, 1997, 20 (3-4) :291-303
[4]  
Bidnenko E, 1998, APPL ENVIRON MICROB, V64, P3059
[5]   The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp lactis IL1403 [J].
Bolotin, A ;
Wincker, P ;
Mauger, S ;
Jaillon, O ;
Malarme, K ;
Weissenbach, J ;
Ehrlich, SD ;
Sorokin, A .
GENOME RESEARCH, 2001, 11 (05) :731-753
[6]   MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE MAJOR PEPTIDOGLYCAN HYDROLASE OF LACTOCOCCUS-LACTIS, A MURAMIDASE NEEDED FOR CELL-SEPARATION [J].
BUIST, G ;
KOK, J ;
LEENHOUTS, KJ ;
DABROWSKA, M ;
VENEMA, G ;
HAANDRIKMAN, AJ .
JOURNAL OF BACTERIOLOGY, 1995, 177 (06) :1554-1563
[7]  
BUIST G, 1997, THESIS U GRONINGEN N
[8]   ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].
CASADABAN, MJ ;
COHEN, SN .
JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) :179-207
[9]   BACTERIAL BIOFILMS IN NATURE AND DISEASE [J].
COSTERTON, JW ;
CHENG, KJ ;
GEESEY, GG ;
LADD, TI ;
NICKEL, JC ;
DASGUPTA, M ;
MARRIE, TJ .
ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :435-464
[10]   Bap, a Staphylococcus aureus surface protein involved in biofilm formation [J].
Cucarella, C ;
Solano, C ;
Valle, J ;
Amorena, B ;
Lasa, I ;
Penadés, JR .
JOURNAL OF BACTERIOLOGY, 2001, 183 (09) :2888-2896