Bio-beads: An efficient strategy for two-dimensional crystallization of membrane proteins

被引：157

作者：

Rigaud, JL

Mosser, G

Lacapere, JJ

Olofsson, A

Levy, D

Ranck, JL

机构：

[1] CEA,LRC 8,F-75231 PARIS,FRANCE

[2] CNRS,CTR GENET MOL,F-91190 GIF SUR YVETTE,FRANCE

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1997年 / 118卷 / 03期

关键词：

D O I：

10.1006/jsbi.1997.3848

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This work establishes the potential of Bio-Beads as a simple alternative to conventional dialysis for removing detergent and for obtaining 2D crystals of integral membrane proteins useful for structure analysis by electron crystallography. Kinetic and equilibrium aspects of removal of different detergents by adsorption onto hydrophobic Bio-Beads SM2 leave been systematically investigated and extended to 2D crystallization of different prototypic membrane proteins, including: (a) Ca2+ ATPase from sarcoplasmic reticulum; (b) melibiose permease from Escherichia coli; (c) cytochrome b(6)f from Chlamydomonas reinhardtii. Different crystals could be produced from all protein preparations, with optical diffraction down to 20-25 Angstrom in negative stain, (C) 1997 Academic Press.

引用

页码：226 / 235

页数：10

共 30 条

[1] Three-dimensional structure of bovine cytochrome bc(1) complex by electron cryomicroscopy and helical image reconstruction
Akiba, T
Toyoshima, C
Matsunaga, T
Kawamoto, M
Kubota, T
Fukuyama, K
Namba, K
Matsubara, H
[J]. NATURE STRUCTURAL BIOLOGY, 1996, 3 (06): : 553 - 561
[2] THE STRUCTURE OF PHOTOSYSTEM-I FROM THE THERMOPHILIC CYANOBACTERIUM SYNECHOCOCCUS SP DETERMINED BY ELECTRON-MICROSCOPY OF 2-DIMENSIONAL CRYSTALS
BOTTCHER, B
GRABER, P
BOEKEMA, EJ
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1100 (02) : 125 - 136
[3] REMOVAL OF TRITON X-100 FROM AQUEOUS-SOLUTION USING AMBERLITE XAD-2
CHEETHAM, PSJ
[J]. ANALYTICAL BIOCHEMISTRY, 1979, 92 (02) : 447 - 452
[4] CLADERA J, 1997, IN PRESS EUR J BIOCH
[5] Some new structural aspects and old controversies concerning the cytochrome b(6)f complex of oxygenic photosynthesis
Cramer, WA
Soriano, GM
Ponomarev, M
Huang, D
Zhang, H
Martinez, SE
Smith, JL
[J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1996, 47 : 477 - 508
[6] 2-D STRUCTURE OF THE NEUROSPORA-CRASSA PLASMA-MEMBRANE ATPASE AS DETERMINED BY ELECTRON CRYOMICROSCOPY
CYRKLAFF, M
AUER, M
KUHLBRANDT, W
SCARBOROUGH, GA
[J]. EMBO JOURNAL, 1995, 14 (09) : 1854 - 1857
[7] The micelle to vesicle transition of lipids and detergents in the presence of a membrane protein: Towards a rationale for 2D crystallization
Dolder, M
Engel, A
Zulauf, M
[J]. FEBS LETTERS, 1996, 382 (1-2): : 203 - 208
[8] ASSEMBLY OF 2-D MEMBRANE-PROTEIN CRYSTALS - DYNAMICS, CRYSTAL ORDER, AND FIDELITY OF STRUCTURE-ANALYSIS BY ELECTRON-MICROSCOPY
ENGEL, A
HOENGER, A
HEFTI, A
HENN, C
FORD, RC
KISTLER, J
ZULAUF, M
[J]. JOURNAL OF STRUCTURAL BIOLOGY, 1992, 109 (03) : 219 - 234
[9] MODEL FOR THE STRUCTURE OF BACTERIORHODOPSIN BASED ON HIGH-RESOLUTION ELECTRON CRYOMICROSCOPY
HENDERSON, R
BALDWIN, JM
CESKA, TA
ZEMLIN, F
BECKMANN, E
DOWNING, KH
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 213 (04) : 899 - 929
[10] SIMPLE PROCEDURE FOR REMOVAL OF TRITON X-100 FROM PROTEIN SAMPLES
HOLLOWAY, PW
[J]. ANALYTICAL BIOCHEMISTRY, 1973, 53 (01) : 304 - 308

← 1 2 3 →