Two-photon excitation microfluorometer for multiplexed single-step bioaffinity assays

被引：32

作者：

Soini, JT ^{[1
]}

Soukka, JM ^{[1
]}

Soini, E ^{[1
]}

Hänninen, PE ^{[1
]}

机构：

[1] Turku Univ, Inst Biomed, Biophys Lab, FIN-20521 Turku, Finland

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2002年 / 73卷 / 07期

关键词：

D O I：

10.1063/1.1485782

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A new type of instrumentation for single-step bioaffinity assays and microvolume fluorometry is presented. The concept is based on the use of two-photon excitation by a low-cost near-infrared laser and individual observation of bioactive fluorescent microparticles. The applicability of the instrument is demonstrated by a microparticle based multiplexed bioaffinity assay where several fluorescent markers are simultaneously excited. This instrument can be applied in the growing fields of drug discovery, in life science research, and in routine laboratory diagnostics. (C) 2002 American Institute of Physics.

引用

页码：2680 / 2685

页数：6

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