Initiation of genome replication: assembly and disassembly of replication-competent chromatin

被引:50
作者
Ritzi, M [1 ]
Knippers, R [1 ]
机构
[1] Univ Konstanz, Dept Biol, D-78457 Constance, Germany
关键词
eukaryotic DNA replication; Mcm proteins; Orc proteins; origins;
D O I
10.1016/S0378-1119(00)00020-2
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Considerable progress has been made in research on the initiation of eukaryotic genome replication. This has generated a number of recent review articles. Here, we briefly summarize the major conclusions described in these articles and also include the results of more recent primary articles. The consensus view that has emerged is that a pre-replication complex assembles during the G1 phase of the cell cycle, making chromatin competent for replication. The complex consists of Ore proteins, Cdc6p, and the family of Mcm proteins. Chromatin, thus 'licenced' for replication, is guided into the S phase by the activation of cell-cycle-regulated protein kinases. Upon entry into S phase, the pre-replication complex is partially dissolved, first by the dissociation of CdcGp and then by the gradual release of Mcm proteins. This appears to be accompanied by a recruitment of chain elongation factors and the establishment of replication forks. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:13 / 20
页数:8
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