Endonuclease-sensitive regions of human spermatozoal chromatin are highly enriched in promoter and CTCF binding sequences

被引:204
作者
Arpanahi, Ali [5 ]
Brinkworth, Martin [2 ]
Iles, David [1 ]
Krawetz, Stephen A. [3 ]
Paradowska, Agnieszka [4 ]
Platts, Adrian E. [3 ]
Saida, Myriam [5 ]
Steger, Klaus [4 ]
Tedder, Philip [6 ]
Miller, David [5 ]
机构
[1] Univ Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
[2] Univ Bradford, Sch Life Sci, Bradford BD7 1DP, W Yorkshire, England
[3] Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Ctr Mol Med & Genet, Detroit, MI 48201 USA
[4] Univ Giessen, Dept Urol & Pediat Urol, D-35385 Giessen, Germany
[5] Univ Leeds, Leeds Inst Genet & Hlth Therapeut, Reprod & Early Dev Unit, Leeds LS2 9JT, W Yorkshire, England
[6] Univ Leeds, Inst Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England
基金
英国生物技术与生命科学研究理事会;
关键词
HUMAN SPERM NUCLEUS; MOUSE MALE GERMLINE; DNA METHYLATION; HUMAN GENOME; PATERNAL CHROMATIN; PROTEIN; ORGANIZATION; PROTAMINE; CELLS; INSULATOR;
D O I
10.1101/gr.094953.109
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During the haploid phase of mammalian spermatogenesis, nucleosomal chromatin is ultimately repackaged by small, highly basic protamines to generate an extremely compact, toroidal chromatin architecture that is critical to normal spermatozoal function. In common with several species, however, the human spermatozoon retains a small proportion of its chromatin packaged in nucleosomes. As nucleosomal chromatin in spermatozoa is structurally more open than protamine-packaged chromatin, we considered it likely to be more accessible to exogenously applied endonucleases. Accordingly, we have used this premise to identify a population of endonuclease-sensitive DNA sequences in human and murine spermatozoa. Our results show unequivocally that, in contrast to the endonuclease-resistant sperm chromatin packaged by protamines, regions of increased endonuclease sensitivity are closely associated with gene regulatory regions, including many promoter sequences and sequences recognized by CCCTC-binding factor (CTCF). Similar differential packaging of promoters is observed in the spermatozoal chromatin of both mouse and man. These observations imply the existence of epigenetic marks that distinguish gene regulatory regions in male germ cells and prevent their repackaging by protamines during spermiogenesis. The ontology of genes under the control of endonuclease-sensitive regulatory regions implies a role for this phenomenon in subsequent embryonic development.
引用
收藏
页码:1338 / 1349
页数:12
相关论文
共 61 条
[1]   Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed [J].
Alexander, Mary Kate ;
Mlynarczyk-Evans, Susanna ;
Royce-Tolland, Morgan ;
Plocik, Alex ;
Kalantry, Sundeep ;
Magnuson, Terry ;
Panning, Barbara .
JOURNAL OF CELL BIOLOGY, 2007, 179 (02) :269-276
[2]  
[Anonymous], 1999, WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction
[3]   MOUSE SPERM CHROMATIN PROTEINS - QUANTITATIVE ISOLATION AND PARTIAL CHARACTERIZATION [J].
BALHORN, R ;
GLEDHILL, BL ;
WYROBEK, AJ .
BIOCHEMISTRY, 1977, 16 (18) :4074-4080
[4]  
Balhorn R, 2000, MOL REPROD DEV, V56, P230, DOI 10.1002/(SICI)1098-2795(200006)56:2+<230::AID-MRD3>3.0.CO
[5]  
2-V
[6]  
Bench G, 1998, MOL REPROD DEV, V50, P345, DOI 10.1002/(SICI)1098-2795(199807)50:3<345::AID-MRD11>3.0.CO
[7]  
2-3
[8]  
Bench GS, 1996, CYTOMETRY, V23, P263, DOI 10.1002/(SICI)1097-0320(19960401)23:4<263::AID-CYTO1>3.3.CO
[9]  
2-9
[10]   Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project [J].
Birney, Ewan ;
Stamatoyannopoulos, John A. ;
Dutta, Anindya ;
Guigo, Roderic ;
Gingeras, Thomas R. ;
Margulies, Elliott H. ;
Weng, Zhiping ;
Snyder, Michael ;
Dermitzakis, Emmanouil T. ;
Stamatoyannopoulos, John A. ;
Thurman, Robert E. ;
Kuehn, Michael S. ;
Taylor, Christopher M. ;
Neph, Shane ;
Koch, Christoph M. ;
Asthana, Saurabh ;
Malhotra, Ankit ;
Adzhubei, Ivan ;
Greenbaum, Jason A. ;
Andrews, Robert M. ;
Flicek, Paul ;
Boyle, Patrick J. ;
Cao, Hua ;
Carter, Nigel P. ;
Clelland, Gayle K. ;
Davis, Sean ;
Day, Nathan ;
Dhami, Pawandeep ;
Dillon, Shane C. ;
Dorschner, Michael O. ;
Fiegler, Heike ;
Giresi, Paul G. ;
Goldy, Jeff ;
Hawrylycz, Michael ;
Haydock, Andrew ;
Humbert, Richard ;
James, Keith D. ;
Johnson, Brett E. ;
Johnson, Ericka M. ;
Frum, Tristan T. ;
Rosenzweig, Elizabeth R. ;
Karnani, Neerja ;
Lee, Kirsten ;
Lefebvre, Gregory C. ;
Navas, Patrick A. ;
Neri, Fidencio ;
Parker, Stephen C. J. ;
Sabo, Peter J. ;
Sandstrom, Richard ;
Shafer, Anthony .
NATURE, 2007, 447 (7146) :799-816