Effects of atrial and brain natriuretic peptides on lysophosphatidylcholine-mediated endothelial dysfunction

被引:17
作者
Murohara, T
Kugiyama, K
Ota, Y
Doi, H
Ogata, N
Ohgushi, M
Yasue, H
机构
[1] Kumamoto Univ, Sch Med, Div Cardiol, Dept Cardiol, Kumamoto 860, Japan
[2] Tufts Univ, Sch Med, St Elizabeths Med Ctr, Dept Cardiol, Boston, MA 02111 USA
关键词
endothelium; nitric oxide; lysolipid; atherosclerosis; signal transduction;
D O I
10.1097/00005344-199912000-00015
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Lysophosphatidylcholine (LPC), a major atherogenic lysophospholipid contained in oxidized low-density lipoprotein (ox-LDL), induces endothelial dysfunction. Recent studies showed that natriuretic peptides (NPs) have antiatherogenic properties by inhibiting vascular smooth-muscle cell proliferation, but their effects on endothelial cells are little known. We examined whether atrial and brain NPs (ANP and BNP) have a protecting action against LPC-induced endothelial dysfunction. LPC (10 mu M) significantly inhibited thrombin (0.001-1 U/ml)-induced endothelium-dependent relaxation without affecting endothelium-independent relaxation to sodium nitroprusside in isolated porcine coronary arteries. The impaired endothelium-dependent relaxation induced by LPC was prevented by treatment with ANP or BNP (1 mu M) In cultured bovine aortic endothelial cells (BAECs), LPC (10 mu M) significantly attenuated bradykinin (1 mu M)-stimulated nitric oxide (NO) release; however, this was prevented by ANP and BNP. Because LPC-induced endothelial dysfunction has been shown to be mediated at least in part by activation of the protein kinase C (PKC)-dependent signaling pathway, wt? also examined the effects of ANP and BNP on LPC-induced modulation of PKC activities in BAECs. LPC (10 mu M) significantly stimulated PKC activity in BAECs. However, ANP or BNP significantly inhibited LPC (10 mu M)-induced PKC activation. In conclusion, ANP and BNP protected endothelial cells from LPC-induced dysfunction in both isolated coronary arteries and cultured ECs. The mechanism appears to be at least in part related to the inhibition of LPC-induced PKC activation by NPs. These new actions of ANP and BNP against lysolipid-induced endothelial cytotoxicity may partly account for antiatherogenic propel ties of NPs.
引用
收藏
页码:870 / 878
页数:9
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