Canalization: what the flux?

Polarized transport of the hormone auxin plays crucial roles in many processes in plant development. A self organizing pattern of auxin transport canalization is thought to be responsible for vascular patterning and shoot branching regulation in flowering plants. Mathematical modeling has demonstrated that membrane localization of PIN-FORMED (PIN)-family auxin efflux carriers in proportion to net auxin flux can plausibly explain canalization and possibly other auxin transport phenomena. Other plausible models have also been proposed, and there has recently been much interest in producing a unified model of all auxin transport phenomena. However, it is our opinion that lacunae in our understanding of auxin transport biology are now limiting progress in developing the next generation of models. Here we examine several key areas where significant experimental advances are necessary to address both biological and theoretical aspects of auxin transport, including the possibility of a unified transport model.