Direct visualization of the microtubular cytoskeleton of ciliated protozoa with a fluorescent taxoid

被引：21

作者：

Arregui, L ^{[1
]}

Muñoz-Fontela, C

Serrano, S

Barasoain, I

Guinea, A

机构：

[1] Univ Complutense, Fac Biol, Dept Microbiol 3, E-28040 Madrid, Spain

[2] Consejo Super Invest Cient, Ctr Invest Biol, Madrid 28006, Spain

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 2002年 / 49卷 / 04期

关键词：

fluorescent Paclitaxel; FLUTAX; identification of ciliates; microtubules; staining methods; taxonomy;

D O I：

10.1111/j.1550-7408.2002.tb00376.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Visualization of the infraciliature, which is an essential tool for the identification of ciliate species, has traditionally been obtained with silver proteinate methods. Since infraciliature is mainly composed of microtubules, we used the synthetic fluorescent taxoid FLUTAX as a method for ciliate identification. The main advantages of this method are the facility and rapidity of its application and the fact that no previous fixation and permeabilization processes are required. FLUTAX may also be used as a probe to follow morphogenetical changes in the microtubular cytoskeleton during the ciliate life cycle.

引用

页码：312 / 318

页数：7

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