Three of four pseudoknots in tmRNA are interchangeable and are substitutable with single-stranded RNAs

被引:60
作者
Nameki, N
Tadaki, T
Himeno, H
Muto, A [1 ]
机构
[1] Hirosaki Univ, Fac Sci, Dept Biol, Hirosaki, Aomori 0368561, Japan
[2] Hirosaki Univ, Fac Agr & Life Sci, Dept Biochem & Biotechnol, Hirosaki, Aomori 0368561, Japan
基金
日本学术振兴会;
关键词
trans-translation; 10Sa RNA; tmRNA; pseudoknot; translation; Escherichia coli;
D O I
10.1016/S0014-5793(00)01349-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel translation, bans-translation, is facilitated by a highly structured RNA molecule, tmRNA, This molecule has two structural domains, a tRNA domain and an mRNA domain, the latter including four pseudoknot structures (PK1 to PK4), Here, we show that replacement of each of these pseudoknots, except PK1, in Escherichia coli tmRNA with a single stranded RNA did not seriously affect the functions as an alanine tRNA and as an mRNA, Furthermore, these three pseudoknots were interchangeable with only small losses of the two functions. These findings suggest that neither PK2, PK3 nor PK4 interacts in a functional manner with ribosome during the trans-translation process, Together with an earlier study showing the significance of PK1, it is concluded that among the four pseudoknots, PK1 is the most functional. (C) 2000 Federation of European Biochemical Societies.
引用
收藏
页码:345 / 349
页数:5
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