Protein destabilizing agents induce polyreactivity and enhanced immunomodulatory activity in IVIg preparations

被引：15

作者：

Djoumerska-Alexieva, Iglika K. ^{[1
]}

Dimitrov, Jordan D. ^{[1
,2
,3
]}

Nacheva, Julia ^{[4
]}

Kaveri, Srini V. ^{[2
,3
]}

Vassilev, Tchavdar L. ^{[1
]}

机构：

[1] Bulgarian Acad Sci, Stefan Angelov Inst Microbiol, Dept Immunol, BU-1113 Sofia, Bulgaria

[2] Univ Paris 06, Ctr Rech Cordeliers, UMRS 872, Paris, France

[3] Univ Paris 05, Paris, France

[4] BulBio NCIPD Ltd, Sofia 1504, Bulgaria

来源：

AUTOIMMUNITY | 2009年 / 42卷 / 04期

关键词：

IgG; IVIg; immunomodulation; immunotherapy; sepsis;

D O I：

10.1080/08916930902832181

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Normal pooled human IVIg are produced using various blood protein fractionation technologies and as a result they may well differ in their biological properties. We have demonstrated that exposure of IVIg, for a period as short as 15 min, to protein-destabilizing agents like acidic pH, ROS or pro-oxidative ferrous ions dramatically increases the panel of recognized Ag including pro-inflammatory cytokines. We now show that exposure of IVIg to ferrous ions modifies some IgG molecules without denaturating them and enhances the protective activity of the preparation in experimental septic shock.

引用

页码：365 / 367

页数：3

共 7 条

[1] Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG fc [J].

Anthony, Robert M. ;

Nimmerjahn, Falk ;

Ashline, David J. ;

Reinhold, Vernon N. ;

Paulson, James C. ;

Ravetch, Jeffrey V. .

SCIENCE, 2008, 320 (5874) :373-376

[2] Ferrous ions and reactive oxygen species increase antigen-binding and anti-inflammatory activities of immunoglobulin G [J].

Dimitrov, JD ;

Ivanovska, ND ;

Lacroix-Desmazes, S ;

Doltchinkova, VR ;

Kaveri, SV ;

Vassilev, TL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (01) :439-446

[3] Insight into the mechanism of the acquired antibody auto-reactivity [J].

Dimitrov, Jordan D. ;

Lacroix-Desmazes, Sebastien ;

Kaveri, Srinivas V. ;

Vassilev, Tchavdar L. .

AUTOIMMUNITY REVIEWS, 2008, 7 (06) :410-414

[4] The autoreactivity of therapeutic intravenous immunoglobulin (IVIg) preparations depends on the fractionation methods used [J].

Djoumerska, I ;

Tcho'banov, A ;

Pashov, A ;

Vassilev, T .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2005, 61 (04) :357-363

[5] Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation [J].

Kaneko, Yoshikatsu ;

Nimmerjahn, Falk ;

Ravetch, Jeffrey V. .

SCIENCE, 2006, 313 (5787) :670-673

[6] Advances in immunology: Immunomodulation of autoimmune and inflammatory diseases with intravenous immune globulin. [J].

Kazatchkine, MD ;

Kaveri, SV .

NEW ENGLAND JOURNAL OF MEDICINE, 2001, 345 (10) :747-755

[7] Inflammation-induced enhancement of IgG immunoreactivity [J].

Mihaylova, N. M. ;

Dimitrov, J. D. ;

Djoumerska-Alexieva, I. K. ;

Vassilev, T. L. .

INFLAMMATION RESEARCH, 2008, 57 (01) :1-3

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