Different neutralization efficiency of neutralizing monoclonal antibodies against avian influenza H5N1 virus to virus strains from different hosts

被引:17
作者
Huang, Hongliang
Dan, Hanbing
Zhou, Yanjun
Yu, Zhengjun
Fan, Huiying
Tong, Tiezhu
Jin, Meilin [1 ]
Chen, Huanchun
机构
[1] Huazhong Normal Univ, Div Anim Infect Dis, Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
[2] Huazhong Normal Univ, Coll Vet Med, Lab Anim Virol, Wuhan 430070, Peoples R China
关键词
avian influenza; H5N1; neutralizing antibody; monoclonal antibody; additivity ELISA;
D O I
10.1016/j.molimm.2005.07.031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
BALB/c mice were immunized with formalin-treated influenza A/CK/Hubei/327/2004 virus. Six monoclonal antibodies specific to HA were selected, designed 1H8, ID11, 2B7, 2C9, 2H4 and 4C9, respectively. The six Mabs probed linear epitopes by western blot assays. In ELISA additivity assays, the low additivity indexes (<= 28.3) of each pair Mabs indicated that the epitopes recognized by the six Mabs were located on the globular head of HA1. The neutralization activity of anti-HA I Mabs and chicken polyclonal sera to various AIV H5N1 strains from different hosts was followed by virus neutralization with MDCK cells. All Mabs except 2C9 and chicken polyclonal serum showed highest neutralizing activity to lowly virulent A/Duck/XF/XFY/2004 from different phylogenetic lineage, and lowest neutralization efficiency to highly virulent A/CK/XF/XFJ/2004. For the other two highly virulent viruses, I D 11, 2H4, 4C9 and chicken polyclonal sera had higher neutralization to A/Goose/ZF/ZFE/2004 than A/CK/Hubei/327/2004, and 1H8 and 2137 had considerable level of neutralizing efficiency to them. These findings suggested that the neutralizing antibodies showed lower neutralization efficiency to highly virulent virus strains than lowly virulent virus strains and strong cross-neutralizing reaction between virus strains located in different phylogenetic lineages. Moreover, the neutralizing Mabs could more efficiently neutralize AIV H5N1 strains from the natural hosts generally, such as waterfowl. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1052 / 1055
页数:4
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