Characterization of d-tagatose-3-epimerase from Rhodobacter sphaeroides that converts d-fructose into d-psicose

被引：125

作者：

Zhang, Longtao ^{[1
]}

Mu, Wanmeng ^{[1
]}

Jiang, Bo ^{[1
]}

Zhang, Tao ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 06期

关键词：

Characterization; D-Fructose; D-Psicose; Rhodobacter sphaeroides; D-Tagatose-3-epimerase; D-TAGATOSE; 3-EPIMERASE; DIETARY D-PSICOSE; RARE SUGAR; METAL-IONS; RATS; 4-EPIMERASE; SYSTEM;

D O I：

10.1007/s10529-009-9942-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A non-characterized gene, previously proposed as the d-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with d-fructose and decreased for other substrates in the order: d-tagatose, d-psicose, d-ribulose, d-xylulose and d-sorbose. Its activity was maximal at pH 9 and 40A degrees C while being enhanced by Mn2+. At pH 9 and 40A degrees C, 118 g d-psicose l(-1) was produced from 700 g d-fructose l(-1) after 3 h.

引用

页码：857 / 862

页数：6

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