Effect of binding and conformation on fluorescence quenching in new 2′,7′-dichlorofluorescein derivatives

被引：39

作者：

Sparano, BA ^{[1
]}

Shahi, SP ^{[1
]}

Koide, K ^{[1
]}

机构：

[1] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA

来源：

ORGANIC LETTERS | 2004年 / 6卷 / 12期

关键词：

D O I：

10.1021/ol049537y

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Symmetrical and unsymmetrical 2',7'-dichlorofluorescein (DCF) derivatives have been synthesized by means of Mannich reactions and an aromatic Claisen rearrangement. NMR and fluorescence spectroscopic studies reveal the correlation between the conformations, the photoinduced electron transfer mechanism, and fluorescent intensities of these DCF derivatives. Two quenching nitrogen atoms cooperatively and reversibly suppress the fluorescence of the chromophore.

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页码：1947 / 1949

页数：3

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