A new immune resonance scattering spectral assay for trace fibrinogen with gold nanoparticle label

被引：31

作者：

Jiang, Zhi-Liang ^{[1
]}

Sun, Shuang-Jiao

Liang, Ai-Hui

Liu, Chang-Jiu

机构：

[1] Guilin Univ Technol, Dept Chem & Mat Engn, Guilin 541004, Peoples R China

[2] Guangxi Normal Univ, Sch Environm & Resource Sci, Guilin 541004, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 571卷 / 02期

关键词：

fibrinogen; immune resonance scattering spectral assay; gold nanoparticle label;

D O I：

10.1016/j.aca.2006.04.071

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gold nanoparticles in size of 9.0 nm was prepared by the trisodium citrate and used to label goat anti-human fibrinogen. In the pH 6.2 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold-labeled goat anti-human fibrinogen and fibrinogen took place and the labeled gold nanoparticles were released from the goat anti-human fibrinogen, and the released gold particles aggregated which leaded the resonance scattering intensity at 560 nm (I-560mm) to enhance greatly. The I-560mm is proportional to the fibrinogen concentration in the range from 0.027 to 1.07 mu g mL(-1). The detection limit is 1.14 ng mL(-1). This simple assay was applied to determination of fibrinogen in human plasma, with satisfactory results. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：200 / 205

页数：6

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