Serum amyloid A-derived peptides, present in human rheumatic synovial fluids, induce the secretion of interferon-γ by human CD4+ T-lymphocytes

Serum amyloid A (SAA) is a major acute-phase protein whose biochemical functions remain largely obscure. Human rheumatic synovial fluids were screened by high performance liquid chromatography mass spectrometry for SAA-derived peptides, specifically the sequence AGLPEKY (SAA(98 similar to 104)) which was previously shown to modulate various leukocyte functions. Two such fluids were found to contain a truncated version of SAA(98-104). Synthetic SAA(98-104) and several of its analogs were shown capable of binding isolated human CD4+ T-lymphocytes and stimulating them to produce interferon-gamma, Given the high acute-phase serum level of SAA acid its massive proteolysis by inflammatory related enzymes, SAA-derived peptides may be involved in host defense mechanisms. (C) 2000 Federation of European Biochemical Societies.