Evaluation of IFNα bioavailability by MxA mRNA in HCV patients

被引：18

作者：

Gilli, F

Sala, A

Bancone, C

Salacone, P

Gallo, M

Gaia, E

Bertolotto, A

机构：

[1] Univ Turin, Ctr Sclerosi Multipla, Div Neurol, Azienda Ospedaliera S Luigi, I-10043 Turin, Italy

[2] Univ Turin, Lab Neurobiol Clin, Div Neurol, Azienda Ospedaliera S Luigi, I-10043 Turin, Italy

[3] Azienda Ospedaliera S Luigi, Gastroenterol Unit, Turin, Italy

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2002年 / 262卷 / 1-2期

关键词：

MxA mRNA; quantitative-competitive PCR; IFN beta; IFN alpha therapy; HCV; hepatitis C;

D O I：

10.1016/S0022-1759(02)00008-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, we have reported the development of a new quantitative-competitive polymerase chain reaction (qc-PCR) method to evaluate interferon-beta (IFNbeta) bioavailability in multiple sclerosis (MS) patients, by measuring mRNA of mixovirus resistance protein A (MxA). Here we show that our assay is also able to assess IFNalpha bioavailability in hepatitis C virus (HCV) patients treated with different IFNalpha regimens. Indeed, our method was able to detect a slight constitutive expression of MxA mRNA in untreated HCV patients (median=70 fgMxA/pgGAPDH) and a significant induction 12 h after the first IFNalpha administration (median=750 fgMxA/pgGAPDH). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：187 / 190

页数：4

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