MsiK-dependent trehalose uptake in Streptomyces reticuli

被引:14
作者
Schlösser, A [1 ]
机构
[1] Univ Osnabruck, FB Biol Chem, D-49069 Osnabruck, Germany
关键词
Streptomyces reticuli; Streptomyces lividans; binding protein-dependent ABC transporter; trehalose uptake; mstK gene; lipoprotein;
D O I
10.1111/j.1574-6968.2000.tb09012.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During cultivation in the presence of trehalose Streptomyces reticuli expresses an inducible. highly specific trehalose uptake system that is absent in Streptomyces lividans. A palmitated trehalose-binding protein was identified in the cytoplasmic membrane of mycelia, extracted with the detergent Triton X-100 and purified using a trehalose affinity matrix. Immunological studies showed that within S. reticuli the synthesis of the ATP-binding protein MsiK is induced by trehalose. The data suggest that MsiK assists the trehalose ABC transporter, like the previously described ABC transport systems for maltose and cellobiose/cellotriose, respectively. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:187 / 192
页数:6
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