Determination of nitrite and nitrate in dairy products by ion exchange LC with spectrophotometric detection

被引：56

作者：

Gapper, LW

Fong, BY

Otter, DE

Indyk, HE

Woollard, DC

机构：

[1] Fonterra, Waitoa, New Zealand

[2] Fonterra Res Ctr, Palmerston North, New Zealand

[3] AgriQual NZ Ltd, Auckland, New Zealand

来源：

INTERNATIONAL DAIRY JOURNAL | 2004年 / 14卷 / 10期

关键词：

nitrite; nitrate; milk; dairy; ion exchange; chromatography;

D O I：

10.1016/j.idairyj.2004.02.015

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An isocratic, ion-exchange chromatographic method is described for the determination of nitrite and nitrate in dairy products. Following dissolution and molecular weight centrifugation (30 kDa), the supernatant is injected directly on to the column and analytes determined at 540 nm subsequent to coupled post-column reduction of nitrate and derivatisation via Griess chemistry. Optimisation and validation studies are presented, including comparison against conventional methods in common usage. Attributes of the described technique include enhanced sensitivity and freedom from endogenous UV-absorbing matrix interferences that compromise an unequivocal chromatographic analysis of infant formulas and protein hydrolysates. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：881 / 887

页数：7

共 24 条

[1] A heuristic derivation of the Horwitz curve [J].